MC Chem Wiki - User contributions [en]

Alan Kuhlemier Chem430 S17

2017-01-20T21:13:21Z

Mgulley: Created page with "Chemistry Research 430 :Spring 2017 :Alan Kuhlemier :Senior Chemistry Major ==Research Times== :? : section 02 = 0.50 credit = 8 hours per week. ==Proposed Research Project=..."

Chemistry Research 430
:Spring 2017
:Alan Kuhlemier
:Senior Chemistry Major

==Research Times==
:?
: section 02 = 0.50 credit = 8 hours per week.

==Proposed Research Project==
===Isolation of Chlorogenic Acid from green coffee beans===
===General Information===
:Advisor: Brad Sturgeon

===Proposal===

[[Chlorogenic_acid_isolation‎|Chlorogenic acid Isolation‎]] from green coffee beans using a [[Ball_mill|ball mill]], and a methanol/water mixture. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

===Instruments to be used===
CombiFlash RF 200i

===References (2 minimum)===
References
:[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]
:[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

===Research pledge===
I, Alan Kuhlemier, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Rachel Book Chem430 S17

2017-01-20T21:11:30Z

Mgulley: Created page with "Chemistry/Biochemistry Research 430 :Spring 2017 :Rachel Book :Sophomore Biochemistry Major ==Research Times== :Mondays 2-5 pm : section 01 = 0.25 credit = 4 hours per week...."

Chemistry/Biochemistry Research 430
:Spring 2017
:Rachel Book
:Sophomore Biochemistry Major

==Research Times==
:Mondays 2-5 pm
: section 01 = 0.25 credit = 4 hours per week.

==General Information==
:Advisor: Audra Sostarecz
:Other research student collaborators: Morgan Gulley, David
:Other Research Collaborators: NONE

==Proposed Research Project==
===Langmuir Monolayer and AFM Analysis of a DPPC (currently)===

:Langmuir-Blodgett Monolayers of Alzheimer's cells. The Langmuir Monolayer technique allows for the analysis of the organization of ampaphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. Currently I am still learning the process of using the Langmuir-Blodgett Monolayer trough; however, once I have gather the technique, I plan to continue David's research with Alzheimer's Disease.

==Instruments to be used==
:Langmuir-Blodgett Monolayer Trough
:ezAFM

==References==
:Ouyang Jiangbo and Lever, A. B. P. "In-trough Langmuir-Blodgett monolayer spectroelectrochemistry of silver (II) tentraneopentoxyphithalocyanine." Journal of Physical Chemistry 95, no. 6 (1991): 2101-2103.

: Triulzi, Robert C., Li, Changquing, Naistat, David, Orbulescu, Jhony, and Leblanc, Roger M. "A Two-Dimensional Approach to Study Amyloid B-Peptide Fragment (23-35)." Journal of Physical Chemistry 111 no. 12 (2007): 4661-4666.

==Research pledge==
I, Rachel Book, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor, Audra Sostarecz.

Brittney Book BIOC430 S17

2017-01-20T21:10:08Z

Mgulley: Created page with "Chemistry/Biochemistry Research 430 :Spring 2017 :Brittney Book :Junior Biochemistry Major ==Research Times== Monday 2-5pm : section 01 = 0.25 credit = 4 hours per week. ==P..."

Chemistry/Biochemistry Research 430
:Spring 2017
:Brittney Book
:Junior Biochemistry Major

==Research Times==
Monday 2-5pm
: section 01 = 0.25 credit = 4 hours per week.

==Proposed Research Project==
===Examining the Effects of Propolis on Cancer Cell Membranes and Bacterial Cell Membranes===

===General Information===
:Advisor: Audra Sostarecz
:Other research student collaborators: Ben, Kelly, Nichole Musselman, and Izabela Sartori
:Other Research Collaborators: Colorado State University, Prof. Debbie Crans

===Abstract===
Langmuir-Blodgett Monolayers of propolis and phospholipids can be examined for antibacterial and anti-cancerous properties. The Langmuir Monolayer technique allows for the analysis of the organization of amphiphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. What is propolis? Propolis is a green-yellow to red-brown resinous material collected from various vegetation around the hive and used to cover the walls of the hive, keep out intruders, and keep out harmful pathogens. There are different chemical composition of the propolis based on the vegetation at the geological location. As a result the biological activity of the propolis is related to the plants native to the site of collection (toreti, 2013). Langmuir monolayers of American propolis are found to have a higher degree of order (less fluidity) and to be more stable indicated by a higher surface pressure at low molecular areas when incorporated into a film with phospholipids such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE).

===Instruments to be used===
:Kibron Trough

===References (2 minimum)===
:Toreti, V. C., Sato H. H., Pastore G. M., & Park Y. K. (2013). Recent Progress of Propolis for Its Biological and Chemical Compositions and Its Botanical Origin. Review Article, 2013, 13.

:Naramoto K., Massashi K., & Ichihara K. (2014). Effects of an Ethanol Exact of Brazilian Green Propolis on Human Cytochrome P450 Enzyme Activities in Vitro. Journal of Agricultural and Food Chemistry, 2014, 62.

:Savka M. A., Dailey L., Popova M., Mihaylova R., Merritt B., Masek M., Le P., Nor S. R., Ahmad M., Hudson A. O., & Bankova V. (2015).Chemical Composition and Disruption of Quorum Sensing Signaling in Geographically Diverse United States Propolis. Review Article, 2015.

===Research pledge===
I, Brittney Book, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Morgan Gulley Chem430 S17

2017-01-20T21:05:25Z

Mgulley: Created page with "Chemistry/Biochemistry Research 430 :Spring 2017 :Morgan Gulley :Senior Chemistry Major with Math & Physics Minors ==Research Times== :Thursdays from 12-3 pm in the Trough La..."

Chemistry/Biochemistry Research 430
:Spring 2017
:Morgan Gulley
:Senior Chemistry Major with Math & Physics Minors

==Research Times==
:Thursdays from 12-3 pm in the Trough Lab
: section 01 = 0.25 credit = 4 hours per week.
: Group Meetings at 3 pm on Fridays

==General Information==
:Advisor: Audra Sostarecz
:Other research student collaborators: Khader
:Other Research Collaborators: NONE

==Proposed Research Project==
===Langmuir Monolayer and AFM Analysis of a Collagen/Phospholipid/Titanium Model Membrane System for the Investigation of Osteoblast Affinity to Titanium Rods===

:Langmuir-Blodgett Monolayers of collagen and phospholipids will be used as a model system for the affinity of osteoblasts to titanium rods. The Langmuir Monolayer technique allows for the analysis of the organization of amphiphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. Collagen, Type I from calf skin, will be used as a substitute for human bone and titanium nitride foil will be used as the substrate due to its increased biocompatibility. Langmuir monolayers of collagen are found to have a higher degree of order (less fluidity) and to be more stable indicated by a higher surface pressure at low molecular areas when incorporated into a film with phospholipids such as dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylethanolamine (DPPE). Similarly, transfer ratio data of collagen monolayers to silicon (SiO2) substrates increased in the presence of DPPC and DPPE indicating that this may be a good model system for this investigation. These studies were completed last semester. In preparation for current experiments involving Atomic Force Microscopy analysis of collagen/phospholipid monolayers transferred to titanium nitride foil using dipalmitolyphosphatidylglycerol (DPPG) as the lipid because of its impact on bacterial resistance in the bone cell model membrane, we have determined that we can use the ezAFM for analysis of transferred multilayers of arachidic acid. It was also determined last semester that a liquid expands/liquid condensed phase of DPPC transferred onto SiO2 by AFM analysis. With this information present, the transfer of a collagen/phospholipid monolayer should be possible, and will be tested.

==Instruments to be used==
:Langmuir-Blodgett Monolayer Trough
:ezAFM

==References==
:Mehdi Kazemzadeh-Narbat, Benjamine F.L. Lai, Chuanfan Ding, Jayachandran N. Kizhakkedathu, Robert E.W. Hancock, and Rizhi Wang. “Multilayered Coating on Titanium for Controlled Release of Antimicrobial Peptides for the Prevention of Implant-Associated Infections.” Biomaterials 34 (2013): 5969–77.

:Jeanette Libera, Thomas Pomorski, Oliviera Josimovic-Alasevic, Karl-Gerd Fritsch, and Andreas Herrmann. “Internalization of Phospholipids from the Plasma Membrane of Human Osteoblast Depends on the Lipid Head Group.” Journal of Bone and Mineral Research 14, no. 5 (1999): 690–99.

==Research pledge==
I, Morgan Gulley, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor, Audra Sostarecz.

Rachel Book Chem430 F16

2017-01-20T20:48:43Z

Mgulley: /* General Information */

Chemistry/Biochemistry Research 430
:Fall 2016
:Rachel Book
:Sophomore Biochemistry Major

==Research Times==
:Tuesdays from 1-5 pm in the Trough Lab
: section 01 = 0.25 credit = 4 hours per week.

==General Information==
:Advisor: Audra Sostarecz
:Other research student collaborators: Morgan Gulley, David
:Other Research Collaborators: NONE

==Proposed Research Project==
===Langmuir Monolayer and AFM Analysis of a DPPC (currently)===

:Langmuir-Blodgett Monolayers of Alzheimer's cells. The Langmuir Monolayer technique allows for the analysis of the organization of ampaphilic molecules at an air-water interface and is, therefore, a useful technique for the formation of model cell membranes. Currently I am still learning the process of using the Langmuir-Blodgett Monolayer trough; however, once I have gather the technique, I plan to continue David's research with Alzheimer's Disease.

==Instruments to be used==
:Langmuir-Blodgett Monolayer Trough
:ezAFM

==References==
:Ouyang Jiangbo and Lever, A. B. P. "In-trough Langmuir-Blodgett monolayer spectroelectrochemistry of silver (II) tentraneopentoxyphithalocyanine." Journal of Physical Chemistry 95, no. 6 (1991): 2101-2103.

: Triulzi, Robert C., Li, Changquing, Naistat, David, Orbulescu, Jhony, and Leblanc, Roger M. "A Two-Dimensional Approach to Study Amyloid B-Peptide Fragment (23-35)." Journal of Physical Chemistry 111 no. 12 (2007): 4661-4666.

==Research pledge==
I, Rachel Book, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor, Audra Sostarecz.

2016-04-22T15:50:22Z

ESR Lab Activity

2016-04-21T22:02:35Z

Mgulley: /* Results */

==Introduction==
We are going to collect an ESR spectrum from a series of substituted hydroquinones.

[[File:Hydroquinones.png]]

:A=Hydroquinone (110.11 g/mol)
:B=methylhydroquinone (124.14 g/mol)
:C=2,3-dimethylhydroquinone (138.16 g/mol)

{| class="wikitable"
||Name
||SDS
||Molar Mass
||Sigma Product Number
||Cost
|-
||Hydroquinone
||[[Media:Hydroquinone.pdf|SDS]]
||110.11 g/mol
||[http://www.sigmaaldrich.com/catalog/product/sial/h9003?lang=en&region=US H9003]
||22.90 / 100g
|-
||Methylhydroquinone
||[[Media:Methylhydroquinone.pdf|SDS]]
||124.14 g/mol
||[http://www.sigmaaldrich.com/catalog/product/aldrich/112968?lang=en&region=US 112968]
||59.20 / 250g
|-
||2,3-dimethylhydroquinone
||[[Media:23Dimethylhydroquinone.pdf|SDS]]
||138.16 g/mol
||[http://www.sigmaaldrich.com/catalog/product/aldrich/300756?lang=en&region=US 300756]
||118.00 / 5g
|}

==Experimental==
===Beaker Method===
*Solution A: 1 M NaOH; 1 gram NaOH (39.997 g/mol) into 25 mL EtOH.
*Solution B: 1 M hydroquinone solution.
*Procedure: To 2 ml of 1 M hydroquinone solution add 2-3 drops of solution A. A color change will indicate the reaction has occurred. Quickly transfer colored sample to ESR sample tube, place in ESR spectrometer, tune, and collected data.

===Flow Method===
*Solution A: 0.05 M NaOH; 0.05 grams NaOH (39.997 g/mol) into 25 ml EtOH.
*Solution B: 1 M hydroquinone solution.
*Procedure: Prepare two 60 ml syringes, 1 with solution A and 1 with solution B.
*Degas the syringe that contains solution B
*Attach to double syringe drive
*Turn on double syringe drive that is attached to ESR
*Collect data
[[File:Double syringe drive.jpg]]

Double Syringe Drive

==Results==
'''Hydroquinone'''

[[File:14BZQ.jpg|1000px]]

This is the EPR graph of the hydroquinone

'''Methylhydroquinone'''

[[File:EPR_MeH2Q.jpg|1000px]]

This is the EPR graph of the methylhydroquinone

==Analysis==

==Conclusions==

ESR Lab Activity

2016-04-21T22:02:23Z

Mgulley: /* Results */

==Introduction==
We are going to collect an ESR spectrum from a series of substituted hydroquinones.

[[File:Hydroquinones.png]]

:A=Hydroquinone (110.11 g/mol)
:B=methylhydroquinone (124.14 g/mol)
:C=2,3-dimethylhydroquinone (138.16 g/mol)

{| class="wikitable"
||Name
||SDS
||Molar Mass
||Sigma Product Number
||Cost
|-
||Hydroquinone
||[[Media:Hydroquinone.pdf|SDS]]
||110.11 g/mol
||[http://www.sigmaaldrich.com/catalog/product/sial/h9003?lang=en&region=US H9003]
||22.90 / 100g
|-
||Methylhydroquinone
||[[Media:Methylhydroquinone.pdf|SDS]]
||124.14 g/mol
||[http://www.sigmaaldrich.com/catalog/product/aldrich/112968?lang=en&region=US 112968]
||59.20 / 250g
|-
||2,3-dimethylhydroquinone
||[[Media:23Dimethylhydroquinone.pdf|SDS]]
||138.16 g/mol
||[http://www.sigmaaldrich.com/catalog/product/aldrich/300756?lang=en&region=US 300756]
||118.00 / 5g
|}

==Experimental==
===Beaker Method===
*Solution A: 1 M NaOH; 1 gram NaOH (39.997 g/mol) into 25 mL EtOH.
*Solution B: 1 M hydroquinone solution.
*Procedure: To 2 ml of 1 M hydroquinone solution add 2-3 drops of solution A. A color change will indicate the reaction has occurred. Quickly transfer colored sample to ESR sample tube, place in ESR spectrometer, tune, and collected data.

===Flow Method===
*Solution A: 0.05 M NaOH; 0.05 grams NaOH (39.997 g/mol) into 25 ml EtOH.
*Solution B: 1 M hydroquinone solution.
*Procedure: Prepare two 60 ml syringes, 1 with solution A and 1 with solution B.
*Degas the syringe that contains solution B
*Attach to double syringe drive
*Turn on double syringe drive that is attached to ESR
*Collect data
[[File:Double syringe drive.jpg]]

Double Syringe Drive

==Results==
'''Hydroquinone'''
[[File:14BZQ.jpg|1000px]]

This is the EPR graph of the hydroquinone

'''Methylhydroquinone'''
[[File:EPR_MeH2Q.jpg|1000px]]

This is the EPR graph of the methylhydroquinone

==Analysis==

==Conclusions==

ESR Lab Activity

2016-04-21T22:01:30Z

Mgulley: /* Hydroquinone */

ESR Lab Activity

2016-04-21T22:01:03Z

Mgulley: /* Hydroquinone */

==Introduction==
We are going to collect an ESR spectrum from a series of substituted hydroquinones.

[[File:Hydroquinones.png]]

:A=Hydroquinone (110.11 g/mol)
:B=methylhydroquinone (124.14 g/mol)
:C=2,3-dimethylhydroquinone (138.16 g/mol)

{| class="wikitable"
||Name
||SDS
||Molar Mass
||Sigma Product Number
||Cost
|-
||Hydroquinone
||[[Media:Hydroquinone.pdf|SDS]]
||110.11 g/mol
||[http://www.sigmaaldrich.com/catalog/product/sial/h9003?lang=en&region=US H9003]
||22.90 / 100g
|-
||Methylhydroquinone
||[[Media:Methylhydroquinone.pdf|SDS]]
||124.14 g/mol
||[http://www.sigmaaldrich.com/catalog/product/aldrich/112968?lang=en&region=US 112968]
||59.20 / 250g
|-
||2,3-dimethylhydroquinone
||[[Media:23Dimethylhydroquinone.pdf|SDS]]
||138.16 g/mol
||[http://www.sigmaaldrich.com/catalog/product/aldrich/300756?lang=en&region=US 300756]
||118.00 / 5g
|}

==Experimental==
===Beaker Method===
*Solution A: 1 M NaOH; 1 gram NaOH (39.997 g/mol) into 25 mL EtOH.
*Solution B: 1 M hydroquinone solution.
*Procedure: To 2 ml of 1 M hydroquinone solution add 2-3 drops of solution A. A color change will indicate the reaction has occurred. Quickly transfer colored sample to ESR sample tube, place in ESR spectrometer, tune, and collected data.

===Flow Method===
*Solution A: 0.05 M NaOH; 0.05 grams NaOH (39.997 g/mol) into 25 ml EtOH.
*Solution B: 1 M hydroquinone solution.
*Procedure: Prepare two 60 ml syringes, 1 with solution A and 1 with solution B.
*Degas the syringe that contains solution B
*Attach to double syringe drive
*Turn on double syringe drive that is attached to ESR
*Collect data
[[File:Double syringe drive.jpg]]

Double Syringe Drive

==Results==
=Hydroquinone=
[[File:14BZQ.jpg|1000px]]

This is the EPR graph of the hydroquinone

==Methylhydroquinone==
[[File:EPR_MeH2Q.jpg|1000px]]

This is the EPR graph of the methylhydroquinone

==Analysis==

==Conclusions==