http://205.166.159.208/wiki/api.php?action=feedcontributions&feedformat=atom&user=Kcherry 2026-05-03T11:15:49Z User contributions MediaWiki 1.35.5 http://205.166.159.208/wiki/index.php?title=Notebook&diff=1235 2016-04-28T20:37:16Z

<p>Kcherry: /* After Lab Procedure */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents and header of the page used for lab. Name, date, title of lab, page <br /> <br /> Example: Stu Dious, 8/26/16, Notebook Practice, Page #1<br /> <br /> 2. Put in purpose in your own words. <br /> <br /> Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up 3 tables to help you find your information easier. <br /> <br /> Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique, such as Table 1: Beaker.<br /> <br /> ==Procedure==<br /> <br /> Data Collection (with lab partner)<br /> <br /> <br /> Obtain a 150 mL beaker from your glassware drawer and place it on an electronic “top-loading” balance. You will “tare” the balance, which means you will set the balance to read 0.00 g. <br /> <br /> Using a “squirt bottle” containing reverse osmosis (RO) water, fill the “tared” beaker up to the first volume mark (probably 20 mL). Be sure to account for the meniscus and be as accurate as possible. One partner will record the volume (ex. 20 mL) and the mass to 2 decimal places (ex. 19.60 note “0” IS a number) in their lab notebook data table. Repeat the mass measurement after adding additional liquid up to the next volume marked on the beaker. Stop data collection once you reach 100 mL. You should end up with at least 5 pairs of data points.<br /> <br /> <br /> Return to your lab bench, discard the distilled water, and dry the beaker with a paper towel…consider saving and reusing the paper towel. At this time, transfer data into to all lab partner’s notebooks.<br /> <br /> <br /> You will now repeat the measurement of a liquid two additional times with the following variations:<br /> <br /> <br /> 1) Instead of using the marking on the beaker, you will add 20.00 ml of RO water to the beaker using a graduated cylinder obtained from your drawer. We suggest using a squirt bottle to fill the graduated cylinder 90% to the mark and then use a small beaker from your drawer with RO water and a transfer pipet (plastic dropper) to add the last few drops. Be sure to account for the meniscus and be as accurate as possible. This data collection will require an additional table in your lab notebook.<br /> <br /> <br /> 2) Repeat the procedure that uses the graduated cylinder for an unknown liquid. Be sure to include the description (mainly ID# and color) of the unknown in your lab notebook.<br /> Once you have collected all the 3 data sets and entered all data into your lab notebook, clean/rinse glassware with RO water. NOW, collect one more set of different “unknown” data from another lab group; enter data in your notebook making sure to include the full names of the other group members. <br /> <br /> <br /> ==After Lab Procedure==<br /> <br /> After finishing the experiment, a scatter plot graph (no lines) is needed to be made INDIVIDUALLY (do NOT just print off two copies). After this, the carbon copy of your notebook can be turned in and at this time, you can complete after lab activities, such as WebAssign assignments. <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1231 2016-04-28T20:36:25Z

<p>Kcherry: /* Procedure */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents and header of the page used for lab. Name, date, title of lab, page <br /> <br /> Example: Stu Dious, 8/26/16, Notebook Practice, Page #1<br /> <br /> 2. Put in purpose in your own words. <br /> <br /> Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up 3 tables to help you find your information easier. <br /> <br /> Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique, such as Table 1: Beaker.<br /> <br /> ==Procedure==<br /> <br /> Data Collection (with lab partner)<br /> <br /> <br /> Obtain a 150 mL beaker from your glassware drawer and place it on an electronic “top-loading” balance. You will “tare” the balance, which means you will set the balance to read 0.00 g. <br /> <br /> Using a “squirt bottle” containing reverse osmosis (RO) water, fill the “tared” beaker up to the first volume mark (probably 20 mL). Be sure to account for the meniscus and be as accurate as possible. One partner will record the volume (ex. 20 mL) and the mass to 2 decimal places (ex. 19.60 note “0” IS a number) in their lab notebook data table. Repeat the mass measurement after adding additional liquid up to the next volume marked on the beaker. Stop data collection once you reach 100 mL. You should end up with at least 5 pairs of data points.<br /> <br /> <br /> Return to your lab bench, discard the distilled water, and dry the beaker with a paper towel…consider saving and reusing the paper towel. At this time, transfer data into to all lab partner’s notebooks.<br /> <br /> <br /> You will now repeat the measurement of a liquid two additional times with the following variations:<br /> <br /> <br /> 1) Instead of using the marking on the beaker, you will add 20.00 ml of RO water to the beaker using a graduated cylinder obtained from your drawer. We suggest using a squirt bottle to fill the graduated cylinder 90% to the mark and then use a small beaker from your drawer with RO water and a transfer pipet (plastic dropper) to add the last few drops. Be sure to account for the meniscus and be as accurate as possible. This data collection will require an additional table in your lab notebook.<br /> <br /> <br /> 2) Repeat the procedure that uses the graduated cylinder for an unknown liquid. Be sure to include the description (mainly ID# and color) of the unknown in your lab notebook.<br /> Once you have collected all the 3 data sets and entered all data into your lab notebook, clean/rinse glassware with RO water. NOW, collect one more set of different “unknown” data from another lab group; enter data in your notebook making sure to include the full names of the other group members. <br /> <br /> <br /> ==After Lab Procedure==<br /> <br /> After finishing the experiment, a scatter plot graph (no lines) is needed to be made INDIVIDUALLY (do NOT just print off two copies). After this, the carbon copy of your notebook can be turned in and time to complete after lab activities. <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1223 2016-04-28T20:34:07Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents and header of the page used for lab. Name, date, title of lab, page <br /> <br /> Example: Stu Dious, 8/26/16, Notebook Practice, Page #1<br /> <br /> 2. Put in purpose in your own words. <br /> <br /> Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up 3 tables to help you find your information easier. <br /> <br /> Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique, such as Table 1: Beaker.<br /> <br /> ==Procedure==<br /> <br /> Data Collection (with lab partner)<br /> <br /> <br /> Obtain a 150 mL beaker from your glassware drawer and place it on an electronic “top-loading” balance. You will “tare” the balance, which means you will set the balance to read 0.00 g. <br /> <br /> Using a “squirt bottle” containing reverse osmosis (RO) water, fill the “tared” beaker up to the first volume mark (probably 20 mL). Be sure to account for the meniscus and be as accurate as possible. One partner will record the volume (ex. 20 mL) and the mass to 2 decimal places (ex. 19.60 note “0” IS a number) in their lab notebook data table. Repeat the mass measurement after adding additional liquid up to the next volume marked on the beaker. Stop data collection once you reach 100 mL. You should end up with at least 5 pairs of data points.<br /> <br /> <br /> Return to your lab bench, discard the distilled water, and dry the beaker with a paper towel…consider saving and reusing the paper towel. At this time, transfer data into to all lab partner’s notebooks.<br /> <br /> <br /> You will now repeat the measurement of a liquid two additional times with the following variations:<br /> <br /> <br /> 1) Instead of using the marking on the beaker, you will add 20.00 ml of RO water to the beaker using a graduated cylinder obtained from your drawer. We suggest using a squirt bottle to fill the graduated cylinder 90% to the mark and then use a small beaker from your drawer with RO water and a transfer pipet (plastic dropper) to add the last few drops. Be sure to account for the meniscus and be as accurate as possible. This data collection will require an additional table in your lab notebook.<br /> <br /> <br /> 2) Repeat the procedure that uses the graduated cylinder for an unknown liquid. Be sure to include the description (mainly ID# and color) of the unknown in your lab notebook.<br /> Once you have collected all the 3 data sets and entered all data into your lab notebook, clean/rinse glassware with RO water. NOW, collect one more set of different “unknown” data from another lab group; enter data in your notebook making sure to include the full names of the other group members. <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1221 2016-04-28T20:33:16Z

<p>Kcherry: /* Procedure */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page <br /> <br /> Example: Stu Dious, 8/26/16, Notebook Practice, Page #1<br /> <br /> 2. Put in purpose in your own words. <br /> <br /> Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up 3 tables to help you find your information easier. <br /> <br /> Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique, such as Table 1: Beaker. <br /> <br /> <br /> <br /> ==Procedure==<br /> <br /> Data Collection (with lab partner)<br /> <br /> <br /> Obtain a 150 mL beaker from your glassware drawer and place it on an electronic “top-loading” balance. You will “tare” the balance, which means you will set the balance to read 0.00 g. <br /> <br /> Using a “squirt bottle” containing reverse osmosis (RO) water, fill the “tared” beaker up to the first volume mark (probably 20 mL). Be sure to account for the meniscus and be as accurate as possible. One partner will record the volume (ex. 20 mL) and the mass to 2 decimal places (ex. 19.60 note “0” IS a number) in their lab notebook data table. Repeat the mass measurement after adding additional liquid up to the next volume marked on the beaker. Stop data collection once you reach 100 mL. You should end up with at least 5 pairs of data points.<br /> <br /> <br /> Return to your lab bench, discard the distilled water, and dry the beaker with a paper towel…consider saving and reusing the paper towel. At this time, transfer data into to all lab partner’s notebooks.<br /> <br /> <br /> You will now repeat the measurement of a liquid two additional times with the following variations:<br /> <br /> <br /> 1) Instead of using the marking on the beaker, you will add 20.00 ml of RO water to the beaker using a graduated cylinder obtained from your drawer. We suggest using a squirt bottle to fill the graduated cylinder 90% to the mark and then use a small beaker from your drawer with RO water and a transfer pipet (plastic dropper) to add the last few drops. Be sure to account for the meniscus and be as accurate as possible. This data collection will require an additional table in your lab notebook.<br /> <br /> <br /> 2) Repeat the procedure that uses the graduated cylinder for an unknown liquid. Be sure to include the description (mainly ID# and color) of the unknown in your lab notebook.<br /> Once you have collected all the 3 data sets and entered all data into your lab notebook, clean/rinse glassware with RO water. NOW, collect one more set of different “unknown” data from another lab group; enter data in your notebook making sure to include the full names of the other group members. <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1212 2016-04-28T20:28:14Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page <br /> <br /> Example: Stu Dious, 8/26/16, Notebook Practice, Page #1<br /> <br /> 2. Put in purpose in your own words. <br /> <br /> Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up 3 tables to help you find your information easier. <br /> <br /> Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique, such as Table 1: Beaker. <br /> <br /> <br /> <br /> ==Procedure==<br /> <br /> Data Collection (with lab partner)<br /> <br /> <br /> Obtain a 150 mL beaker from your glassware drawer and place it on an electronic “top-loading” balance. You will “tare” the balance, which means you will set the balance to read 0.00 g. Using a “squirt bottle” containing reverse osmosis (RO) water, fill the “tared” beaker up to the first volume mark (probably 20 mL). Be sure to account for the meniscus and be as accurate as possible. One partner will record the volume (ex. 20 mL) and the mass to 2 decimal places (ex. 19.60 note “0” IS a number) in their lab notebook data table. Repeat the mass measurement after adding additional liquid up to the next volume marked on the beaker. Stop data collection once you reach 100 mL. You should end up with at least 5 pairs of data points.<br /> Return to your lab bench, discard the distilled water, and dry the beaker with a paper towel…consider saving and reusing the paper towel. At this time, transfer data into to all lab partner’s notebooks.<br /> <br /> <br /> You will now repeat the measurement of a liquid two additional times with the following variations:<br /> 1) Instead of using the marking on the beaker, you will add 20.00 ml of RO water to the beaker using a graduated cylinder obtained from your drawer. We suggest using a squirt bottle to fill the graduated cylinder 90% to the mark and then use a small beaker from your drawer with RO water and a transfer pipet (plastic dropper) to add the last few drops. Be sure to account for the meniscus and be as accurate as possible. This data collection will require an additional table in your lab notebook.<br /> 2) Repeat the procedure that uses the graduated cylinder for an unknown liquid. Be sure to include the description (mainly ID# and color) of the unknown in your lab notebook.<br /> Once you have collected all the 3 data sets and entered all data into your lab notebook, clean/rinse glassware with RO water. NOW, collect one more set of different “unknown” data from another lab group; enter data in your notebook making sure to include the full names of the other group members. <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1210 2016-04-28T20:26:33Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page <br /> <br /> Example: Stu Dious, 8/26/16, Notebook Practice, Page #1<br /> <br /> 2. Put in purpose in your own words. <br /> <br /> Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. <br /> <br /> Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique, such as Table 1: Beaker. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1205 2016-04-28T20:24:43Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page (Ex: Stu Dious, 8/26/16, Notebook Practice, Page #1)<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=ESR_Lab_Activity&diff=1202 2016-04-28T20:21:16Z

<p>Kcherry: /* Gaussian Calculations */</p> <hr /> <div>==Introduction==<br /> We are going to collect an ESR spectrum from a series of substituted hydroquinones.<br /> <br /> [[File:Hydroquinones.png]]<br /> <br /> {| class=&quot;wikitable&quot;<br /> ||Name<br /> ||SDS<br /> ||Molar Mass<br /> ||Sigma Product Number<br /> ||Cost<br /> |-<br /> ||A = Hydroquinone (aka. 1,4-benzohydroquinone)<br /> ||[[Media:Hydroquinone.pdf|SDS]]<br /> ||110.11 g/mol<br /> ||[http://www.sigmaaldrich.com/catalog/product/sial/h9003?lang=en&amp;region=US H9003]<br /> ||22.90 / 100g<br /> |-<br /> ||B = Methylhydroquinone<br /> ||[[Media:Methylhydroquinone.pdf|SDS]]<br /> ||124.14 g/mol<br /> ||[http://www.sigmaaldrich.com/catalog/product/aldrich/112968?lang=en&amp;region=US 112968]<br /> ||59.20 / 250g<br /> |-<br /> ||C = 2,3-dimethylhydroquinone<br /> ||[[Media:23Dimethylhydroquinone.pdf|SDS]]<br /> ||138.16 g/mol<br /> ||[http://www.sigmaaldrich.com/catalog/product/aldrich/300756?lang=en&amp;region=US 300756]<br /> ||118.00 / 5g<br /> |}<br /> <br /> ==Experimental==<br /> ===Beaker Method===<br /> *Solution A: 1 M NaOH; 1 gram NaOH (39.997 g/mol) into 25 mL EtOH.<br /> *Solution B: 1 M hydroquinone solution.<br /> *Procedure: To 2 ml of 1 M hydroquinone solution add 2-3 drops of solution A. A color change will indicate the reaction has occurred. Quickly transfer colored sample to ESR sample tube, place in ESR spectrometer, tune, and collected data.<br /> <br /> ===Flow Method=== <br /> *Solution A: 0.05 M NaOH; 0.05 grams NaOH (39.997 g/mol) into 25 ml EtOH.<br /> *Solution B: 1 M hydroquinone solution.<br /> *Procedure: Prepare two 60 ml syringes, 1 with solution A and 1 with solution B.<br /> *Degas the syringe that contains solution B<br /> *Attach to double syringe drive<br /> *Turn on double syringe drive that is attached to ESR<br /> *Collect data<br /> [[File:Double syringe drive.jpg]]<br /> <br /> Double Syringe Drive<br /> <br /> ==Results==<br /> ===1-electron Oxidation of Hydroquinone====<br /> <br /> <br /> [[File:14BZQ.jpg|500px]]<br /> <br /> <br /> This is the EPR spectrum of the &quot;hydro-semiquinone&quot; (aka. 1,4-benzosemiquinone)<br /> <br /> EPR Parameters: 9.4 GHz, 3360 G Center field, 15 G sweep width. [Above data needs the x-axis changed over to magnetic field in G]<br /> <br /> ===1-electron Oxidation of Methylhydroquinone===<br /> <br /> <br /> [[File:EPR_MeH2Q.jpg|500px]]<br /> <br /> <br /> This is the EPR spectrum of the &quot;methyl-hydro-semiquinone&quot; (aka. methyl-semiquinone)<br /> <br /> EPR Parameters: 9.4 GHz, 3360 G Center field, 15 G sweep width. [Above data needs the x-axis changed over to magnetic field in G]<br /> <br /> ==Analysis==<br /> ===WinSim===<br /> ===Gaussian Calculations===<br /> <br /> {|class=&quot;wikitable&quot;<br /> ||Atom<br /> ||HF/3-21G<br /> ||HF/6-31G<br /> ||HF/6311G+(2p,d)<br /> ||B3LYP/6-31G<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||8<br /> ||12.605<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||9<br /> ||-14.289<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||11<br /> ||-14.289<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||12<br /> ||12.604<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||19<br /> ||12.604<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||20<br /> ||-14.289<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||22<br /> ||-14.289<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||23<br /> ||12.604<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||24<br /> ||0.661<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |-<br /> ||25<br /> ||0.660<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> ||<br /> |}<br /> <br /> ==Conclusions==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1088 2016-04-22T15:52:20Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1087 2016-04-22T15:52:13Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1085 2016-04-22T15:52:01Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1083 2016-04-22T15:51:53Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1081 2016-04-22T15:51:42Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1079 2016-04-22T15:51:30Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> List them by the different measuring technique. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1074 2016-04-22T15:50:54Z

<p>Kcherry: /* Prepare your Notebook */</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques (beaker, graduated cylinder, transfer pipet) <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Notebook&diff=1070 2016-04-22T15:49:41Z

<p>Kcherry: Created page with &quot;===Notebook Lab=== ==Prepare your Notebook== 1. Fill out Table of Contents. Name, date, title of lab, page 2. Put in purpose in your own words. Example: To find the relations...&quot;</p> <hr /> <div>===Notebook Lab===<br /> ==Prepare your Notebook==<br /> 1. Fill out Table of Contents. Name, date, title of lab, page<br /> <br /> 2. Put in purpose in your own words. Example: To find the relationship of volume with different measuring techniques <br /> <br /> 3. Data/ Procedure. Set up graphs to help you find your information easier. Example: <br /> <br /> [[File:ExampleGraph.PNG|200px|thumb|left|alt text]]<br /> <br /> [[File:Notebook Lab.pdf]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=File:ExampleGraph.PNG&diff=1059 2016-04-22T15:47:11Z

<p>Kcherry: Example graph for in your notebook</p> <hr /> <div>Example graph for in your notebook</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Free_Radical_Generation&diff=847 2016-04-14T21:39:27Z

<p>Kcherry: /* 1. KMnO4 */</p> <hr /> <div>The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means. <br /> <br /> =='''Chemical Means'''==<br /> <br /> ===1. KMnO4===<br /> [[Media: KMNO4.pdf|KMnO4]]<br /> <br /> To make a 2 mM solution of KMnO4, we added 0.0316g of KMnO4 solid to 100 mL dH2O<br /> <br /> To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O<br /> <br /> ===2. Cerium (IV)===<br /> For Cerium Free Radicals:<br /> 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).<br /> <br /> For 100 mL of 2 mM HPA:<br /> <br /> Weigh out 30.4mg of HPA<br /> <br /> Add it into 100 mL DI water<br /> <br /> For 100 mL of 2 mM Ce (IV)<br /> <br /> Weigh out 109.6 mg of Ammonium Cerium Nitrate<br /> <br /> Add that to 2.5 mL of 18M sulfuric acid<br /> <br /> Add that solution to 97.5 mL of DI water<br /> <br /> ===3. Fenton Chemistry===<br /> [[Media: Fenton.pdf|Fenton Chemistry Article]]<br /> <br /> Fe2+ + H2O2 ---&gt; OH• + phenol ---&gt; phenoxyl radical<br /> <br /> ====Solutions====<br /> 1. HPA and hydrogen peroxide (100mL)<br /> <br /> 2mmolar HPA with 2mmolar hydrogen peroxide<br /> <br /> a. HPA made with 0.0300g of 152.015g/mol HPA<br /> b. 30 microliters of (30%) hydrogen peroxide was added<br /> <br /> 2. Iron(II) (100mL)<br /> <br /> 1M phosphoric acid with 1 milligram of Iron (II)<br /> <br /> a. 6.82mL Phosphoric acid was mixed with 93.18mL of RO water<br /> b. 1 mg of FeSO4 was added<br /> <br /> ====ESR Results====<br /> These results were inconclusive at this time<br /> <br /> ==Enzymatic Means==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Free_Radical_Generation&diff=834 2016-04-14T21:28:00Z

<p>Kcherry: /* 1. KMnO4 */</p> <hr /> <div>The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means. <br /> <br /> =='''Chemical Means'''==<br /> <br /> ===1. KMnO4===<br /> [[Media: KMNO4.pdf|KMnO4]]<br /> <br /> To make a 2 mM solution of KMnO4, we added 0.03g of KMnO4 solid to 100 mL dH2O<br /> <br /> To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O<br /> <br /> ===2. Cerium (IV)===<br /> For Cerium Free Radicals:<br /> 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).<br /> <br /> For 100 mL of 2 mM HPA:<br /> <br /> Weigh out 30.4mg of HPA<br /> <br /> Add it into 100 mL DI water<br /> <br /> For 100 mL of 2 mM Ce (IV)<br /> <br /> Weigh out 109.6 mg of Ammonium Cerium Nitrate<br /> <br /> Add that to 2.5 mL of 18M sulfuric acid<br /> <br /> Add that solution to 97.5 mL of DI water<br /> <br /> ===3. Fenton Chemistry===<br /> [[Media: Fenton.pdf|Fenton Chemistry Article]]<br /> <br /> ====Solutions====<br /> <br /> ==Enzymatic Means==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Free_Radical_Generation&diff=833 2016-04-14T21:27:49Z

<p>Kcherry: /* 1. KMnO4 */</p> <hr /> <div>The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means. <br /> <br /> =='''Chemical Means'''==<br /> <br /> ===1. KMnO4===<br /> [[Media: KMNO4.pdf|KMnO4]]<br /> To make a 2 mM solution of KMnO4, we added 0.03g of KMnO4 solid to 100 mL dH2O<br /> <br /> To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O<br /> <br /> ===2. Cerium (IV)===<br /> For Cerium Free Radicals:<br /> 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).<br /> <br /> For 100 mL of 2 mM HPA:<br /> <br /> Weigh out 30.4mg of HPA<br /> <br /> Add it into 100 mL DI water<br /> <br /> For 100 mL of 2 mM Ce (IV)<br /> <br /> Weigh out 109.6 mg of Ammonium Cerium Nitrate<br /> <br /> Add that to 2.5 mL of 18M sulfuric acid<br /> <br /> Add that solution to 97.5 mL of DI water<br /> <br /> ===3. Fenton Chemistry===<br /> [[Media: Fenton.pdf|Fenton Chemistry Article]]<br /> <br /> ====Solutions====<br /> <br /> ==Enzymatic Means==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Free_Radical_Generation&diff=832 2016-04-14T21:27:37Z

<p>Kcherry: /* Chemical Means */</p> <hr /> <div>The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means. <br /> <br /> =='''Chemical Means'''==<br /> <br /> ===1. KMnO4===<br /> [[Media: KMNO4.pdf|KMnO4]]<br /> To make a 2 mM solution of KMnO4, we added 0.03g of KMnO4 solid to 100 mL dH2O<br /> To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O<br /> <br /> ===2. Cerium (IV)===<br /> For Cerium Free Radicals:<br /> 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).<br /> <br /> For 100 mL of 2 mM HPA:<br /> <br /> Weigh out 30.4mg of HPA<br /> <br /> Add it into 100 mL DI water<br /> <br /> For 100 mL of 2 mM Ce (IV)<br /> <br /> Weigh out 109.6 mg of Ammonium Cerium Nitrate<br /> <br /> Add that to 2.5 mL of 18M sulfuric acid<br /> <br /> Add that solution to 97.5 mL of DI water<br /> <br /> ===3. Fenton Chemistry===<br /> [[Media: Fenton.pdf|Fenton Chemistry Article]]<br /> <br /> ====Solutions====<br /> <br /> ==Enzymatic Means==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Free_Radical_Generation&diff=831 2016-04-14T21:27:22Z

<p>Kcherry: /* 1. KMnO4 */</p> <hr /> <div>The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means. <br /> <br /> =='''Chemical Means'''==<br /> <br /> 1. KMnO4<br /> [[Media: KMNO4.pdf|KMnO4]]<br /> To make a 2 mM solution of KMnO4, we added 0.03g of KMnO4 solid to 100 mL dH2O<br /> To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O<br /> <br /> ===2. Cerium (IV)===<br /> For Cerium Free Radicals:<br /> 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).<br /> <br /> For 100 mL of 2 mM HPA:<br /> <br /> Weigh out 30.4mg of HPA<br /> <br /> Add it into 100 mL DI water<br /> <br /> For 100 mL of 2 mM Ce (IV)<br /> <br /> Weigh out 109.6 mg of Ammonium Cerium Nitrate<br /> <br /> Add that to 2.5 mL of 18M sulfuric acid<br /> <br /> Add that solution to 97.5 mL of DI water<br /> <br /> ===3. Fenton Chemistry===<br /> [[Media: Fenton.pdf|Fenton Chemistry Article]]<br /> <br /> ====Solutions====<br /> <br /> ==Enzymatic Means==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Free_Radical_Generation&diff=830 2016-04-14T21:27:04Z

<p>Kcherry: /* Chemical Means */</p> <hr /> <div>The 1-electron oxidation of a phenol can be accomplished via chemical or enzymatic means. <br /> <br /> =='''Chemical Means'''==<br /> <br /> ==1. KMnO4==<br /> [[Media: KMNO4.pdf|KMnO4]]<br /> To make a 2 mM solution of KMnO4, we added 0.03g of KMnO4 solid to 100 mL dH2O<br /> To make a 2 mM solution of BHA, we added 0.0304 g of solid HPA to 100 mL dH2O<br /> <br /> ===2. Cerium (IV)===<br /> For Cerium Free Radicals:<br /> 100 mL solution of 2 mM HPA and 100 mL solution of Ce (IV).<br /> <br /> For 100 mL of 2 mM HPA:<br /> <br /> Weigh out 30.4mg of HPA<br /> <br /> Add it into 100 mL DI water<br /> <br /> For 100 mL of 2 mM Ce (IV)<br /> <br /> Weigh out 109.6 mg of Ammonium Cerium Nitrate<br /> <br /> Add that to 2.5 mL of 18M sulfuric acid<br /> <br /> Add that solution to 97.5 mL of DI water<br /> <br /> ===3. Fenton Chemistry===<br /> [[Media: Fenton.pdf|Fenton Chemistry Article]]<br /> <br /> ====Solutions====<br /> <br /> ==Enzymatic Means==</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=699 2016-04-07T21:17:34Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]][[File:Tylers mould.png|100px]]|||5|[[File:Ian's_Mixer_Thing.PNG|200px]]|||6|[[File:Kaylas 3D idea.PNG |150px]]|[[File:Kaylas 3D reality.png |150px]]|7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=698 2016-04-07T21:17:14Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]][[File:Tylers mould.png|100px]]|||5|[[File:Ian's_Mixer_Thing.PNG|200px]]|6|[[File:Kaylas 3D idea.PNG |150px]]|[[File:Kaylas 3D reality.png |150px]]|7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=697 2016-04-07T21:16:56Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]][[File:Tylers mould.png|100px]]|||5|[[File:Ian's_Mixer_Thing.PNG|200px]]|6|[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]|7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=696 2016-04-07T21:16:30Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]][[File:Tylers mould.png|100px]]|[[File:Ian's_Mixer_Thing.PNG|200px]]||5||6|[[File:Kaylas 3D idea.PNG |150px]][[File:Kaylas 3D reality.png |150px]]|7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=695 2016-04-07T21:16:17Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]][[File:Tylers mould.png|100px]]|[[File:Ian's_Mixer_Thing.PNG|200px]]||5||6|[[File:Kaylas 3D idea.PNG |200px]][[File:Kaylas 3D reality.png |200px]]|7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=694 2016-04-07T21:15:56Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]][[File:Tylers mould.png|100px]]|[[File:Ian's_Mixer_Thing.PNG|200px]]||5||6|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]|7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=692 2016-04-07T21:14:48Z

<p>Kcherry: /* Flow Cell Experimentation */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> <br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |Rowspan=&quot;2&quot;|[[File:Printer.PNG|200px|thumb|center|Select The Printer in Matter Control]]<br /> ||[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> ||[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Screen Shot 2016-04-07 at 4.01.54 PM.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||[[File:Tylers Tinkercad.PNG |100px]]|[[File:Ian's_Mixer_Thing.PNG|200px]]||5||6||7||8||9||10<br /> |-<br /> |1|[[File:Kaylas 3D idea.PNG |100px]][[File:Kaylas 3D reality.png |100px]]|2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=File:Kaylas_3D_reality.png&diff=690 2016-04-07T21:14:08Z

<p>Kcherry: My 3D cast for a mixer.</p> <hr /> <div>My 3D cast for a mixer.</div>

Kcherry http://205.166.159.208/wiki/index.php?title=File:Kaylas_3D_idea.PNG&diff=687 2016-04-07T21:09:56Z

<p>Kcherry: 3D idea on Tinkercad</p> <hr /> <div>3D idea on Tinkercad</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=678 2016-04-07T21:01:50Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D-printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Siliconecasting.png|600px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=675 2016-04-07T20:59:25Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> [[File:Siliconecasting.png|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=673 2016-04-07T20:57:23Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=672 2016-04-07T20:57:02Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]<br /> <br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=671 2016-04-07T20:56:34Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]<br /> <br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=670 2016-04-07T20:56:00Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]<br /> <br /> <br /> <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=669 2016-04-07T20:55:45Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> <br /> <br /> {|align=&quot;center&quot;[[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]|}<br /> <br /> <br /> <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=668 2016-04-07T20:55:33Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> <br /> <br /> {|align=&quot;center&quot; [[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]|}<br /> <br /> <br /> <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=666 2016-04-07T20:55:18Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> <br /> <br /> {|align=&quot;center&quot; <br /> [[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]<br /> |}<br /> <br /> <br /> <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=665 2016-04-07T20:53:40Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=663 2016-04-07T20:52:50Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> {|align=&quot;center&quot;[[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]|}<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=660 2016-04-07T20:51:54Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> [[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=658 2016-04-07T20:50:11Z

<p>Kcherry: /* Silicon Casting */</p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> |[[File:Cast in desiccator.jpg|200px|thumb|right|Matter Control Layer Settings]]<br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.<br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |200px]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=File:Cast_in_desiccator.jpg&diff=650 2016-04-07T20:47:01Z

<p>Kcherry: A picture showing the 3D printed cast in a desiccator.</p> <hr /> <div>A picture showing the 3D printed cast in a desiccator.</div>

Kcherry http://205.166.159.208/wiki/index.php?title=Flow_Cell_Lab_Activity&diff=649 2016-04-07T20:46:02Z

<p>Kcherry: </p> <hr /> <div>==Introduction to Flow Cells==<br /> [[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]<br /> <br /> The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.<br /> <br /> ==Producing The Virtual Object==<br /> Tinkercad.com<br /> <br /> Sujith<br /> <br /> ==Prep for 3d Printing==<br /> [[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]<br /> A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.<br /> <br /> The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.<br /> {|align=&quot;center&quot;<br /> |[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]<br /> ||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]<br /> ||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]<br /> |-<br /> |[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]<br /> ||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]<br /> ||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]<br /> |}<br /> <br /> ==3d Printing==<br /> <br /> <br /> ==Silicon Casting==<br /> After the 3D cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough. <br /> <br /> <br /> <br /> After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below. <br /> <br /> <br /> <br /> ==Flow Cell Construction==<br /> <br /> <br /> ==Flow Cell Experimentation==<br /> video/camera<br /> results<br /> <br /> {|align=&quot;center&quot;<br /> | Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith<br /> |-<br /> |1||2||[[File:Morgan%27sVirtual.PNG]][[File:Morgan'sReality.PNG]]||4||5||6||7||8||9||10<br /> |-<br /> |1||2||3||4||5||6||7||8||9||10<br /> |}</div>

Kcherry http://205.166.159.208/wiki/index.php?title=FT-IR_(Nicolet)&diff=326 2016-02-25T21:06:32Z

<p>Kcherry: /* Other links of interest */</p> <hr /> <div>== Model== <br /> Thermo Nicolet Nexus 470 FT-IR <br /> <br /> User manual : http://mmrc.caltech.edu/FTIR/Nicolet/Nicolet%20Software/Nicolet%202/4700_6700_User.pdf <br /> <br /> ==Operating Instructions==<br /> ===Solid Samples===<br /> * Open OMNIC software<br /> * Clean the instrument with ethanol and a KimWipe<br /> * Collect background data with laser screwed down (Col Bkg)<br /> * Place sample and screw down the laser<br /> * Col Sample (Col Sam)<br /> * Delete background data by clicking on the background spectrum and hitting the &quot;Clear&quot; Button<br /> * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet <br /> <br /> ~OR page 30-36 on the user manual~<br /> <br /> ===Air samples===<br /> * Set up the air tube for the FTIR<br /> * Collect background of the air in the air tube (Col Bkg)<br /> * Vent out air and allow the air sample to enter the tube<br /> * Collect sample (Col Sam)<br /> * Delete background data by clicking on the background spectrum and hitting the &quot;Clear&quot; Button<br /> * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet<br /> <br /> ===Downloading Data Files===<br /> *Download the .csv<br /> *Load waves into Igor<br /> *Create a graph<br /> *Right click on the x axis numbers and hit swap to make the spectra look identical to the computer's right after the sample was collected<br /> <br /> ==Example Spectra==<br /> [[File:Vinyl.jpeg|400px|thumb|left|FT-IR:Vinyl Spectrum]]<br /> [[File:Polypropylene.jpeg|400px|thumb|left|FT-IR:Polypropylene Spectrum]]<br /> [[File:Polymethylmethacrylate.jpg|400px|thumb|left|FT-IR:Polymethylmethacrylate Spectrum]]<br /> [[File:Polyethylene terephthalate.jpg|400px|thumb|left|FT-IR:Polyethylene terephthalate Spectrum]]<br /> <br /> Links to manufacturer: http://www.gmi-inc.com/thermo-nicolet-nexus-470-ftir.html <br /> <br /> ==Other links of interest==<br /> Fourier transform infrared spectroscopy (FTIR) is a technique which is used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high spectral resolution data over a wide spectral range. This confers a significant advantage over a dispersive spectrometer which measures intensity over a narrow range of wavelengths at a time. Rotational–vibrational spectroscopy is a branch of molecular spectroscopy concerned with infrared and Raman spectra of molecules in the gas phase. Transitions involving changes in both vibrational and rotational states can be abbreviated as rovibrational (or ro-vibrational) transitions. <br /> https://en.wikipedia.org/wiki/Infrared_spectroscopy has some helpful gifs of how to tell if a molecule is FTIR active or not. <br /> <br /> [[[[File:FTIR instrument.png|400px|thumb|left|FTIR instrument]]]]<br /> [[[[File:FTIR_cheatsheet_2.gif|400px|thumb|left|FTIR_cheatsheet]]]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=FT-IR_(Nicolet)&diff=322 2016-02-25T21:04:38Z

<p>Kcherry: /* Other links of interest */</p> <hr /> <div>== Model== <br /> Thermo Nicolet Nexus 470 FT-IR <br /> <br /> User manual : http://mmrc.caltech.edu/FTIR/Nicolet/Nicolet%20Software/Nicolet%202/4700_6700_User.pdf <br /> <br /> ==Operating Instructions==<br /> ===Solid Samples===<br /> * Open OMNIC software<br /> * Clean the instrument with ethanol and a KimWipe<br /> * Collect background data with laser screwed down (Col Bkg)<br /> * Place sample and screw down the laser<br /> * Col Sample (Col Sam)<br /> * Delete background data by clicking on the background spectrum and hitting the &quot;Clear&quot; Button<br /> * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet <br /> <br /> ~OR page 30-36 on the user manual~<br /> <br /> ===Air samples===<br /> * Set up the air tube for the FTIR<br /> * Collect background of the air in the air tube (Col Bkg)<br /> * Vent out air and allow the air sample to enter the tube<br /> * Collect sample (Col Sam)<br /> * Delete background data by clicking on the background spectrum and hitting the &quot;Clear&quot; Button<br /> * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet<br /> <br /> ===Downloading Data Files===<br /> *Download the .csv<br /> *Load waves into Igor<br /> *Create a graph<br /> *Right click on the x axis numbers and hit swap to make the spectra look identical to the computer's right after the sample was collected<br /> <br /> ==Example Spectra==<br /> [[File:Vinyl.jpeg|400px|thumb|left|FT-IR:Vinyl Spectrum]]<br /> [[File:Polypropylene.jpeg|400px|thumb|left|FT-IR:Polypropylene Spectrum]]<br /> [[File:Polymethylmethacrylate.jpg|400px|thumb|left|FT-IR:Polymethylmethacrylate Spectrum]]<br /> [[File:Polyethylene terephthalate.jpg|400px|thumb|left|FT-IR:Polyethylene terephthalate Spectrum]]<br /> <br /> Links to manufacturer: http://www.gmi-inc.com/thermo-nicolet-nexus-470-ftir.html <br /> <br /> ==Other links of interest==<br /> Fourier transform infrared spectroscopy (FTIR) is a technique which is used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. An FTIR spectrometer simultaneously collects high spectral resolution data over a wide spectral range. This confers a significant advantage over a dispersive spectrometer which measures intensity over a narrow range of wavelengths at a time. Rotational–vibrational spectroscopy is a branch of molecular spectroscopy concerned with infrared and Raman spectra of molecules in the gas phase. Transitions involving changes in both vibrational and rotational states can be abbreviated as rovibrational (or ro-vibrational) transitions. <br /> <br /> [[File:FTIR instrument.png|400px|thumb|left|FTIR instrument]]<br /> [[File:FTIR_cheatsheet_2.gif|400px|thumb|left|FTIR_cheatsheet]]</div>

Kcherry http://205.166.159.208/wiki/index.php?title=FT-IR_(Nicolet)&diff=321 2016-02-25T21:02:56Z

<p>Kcherry: </p> <hr /> <div>== Model== <br /> Thermo Nicolet Nexus 470 FT-IR <br /> <br /> User manual : http://mmrc.caltech.edu/FTIR/Nicolet/Nicolet%20Software/Nicolet%202/4700_6700_User.pdf <br /> <br /> ==Operating Instructions==<br /> ===Solid Samples===<br /> * Open OMNIC software<br /> * Clean the instrument with ethanol and a KimWipe<br /> * Collect background data with laser screwed down (Col Bkg)<br /> * Place sample and screw down the laser<br /> * Col Sample (Col Sam)<br /> * Delete background data by clicking on the background spectrum and hitting the &quot;Clear&quot; Button<br /> * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet <br /> <br /> ~OR page 30-36 on the user manual~<br /> <br /> ===Air samples===<br /> * Set up the air tube for the FTIR<br /> * Collect background of the air in the air tube (Col Bkg)<br /> * Vent out air and allow the air sample to enter the tube<br /> * Collect sample (Col Sam)<br /> * Delete background data by clicking on the background spectrum and hitting the &quot;Clear&quot; Button<br /> * Save sample as a .csv file onto a flash drive; our instrument doesn't have internet<br /> <br /> ===Downloading Data Files===<br /> *Download the .csv<br /> *Load waves into Igor<br /> *Create a graph<br /> *Right click on the x axis numbers and hit swap to make the spectra look identical to the computer's right after the sample was collected<br /> <br /> ==Example Spectra==<br /> [[File:Vinyl.jpeg|400px|thumb|left|FT-IR:Vinyl Spectrum]]<br /> [[File:Polypropylene.jpeg|400px|thumb|left|FT-IR:Polypropylene Spectrum]]<br /> [[File:Polymethylmethacrylate.jpg|400px|thumb|left|FT-IR:Polymethylmethacrylate Spectrum]]<br /> [[File:Polyethylene terephthalate.jpg|400px|thumb|left|FT-IR:Polyethylene terephthalate Spectrum]]<br /> <br /> Links to manufacturer: http://www.gmi-inc.com/thermo-nicolet-nexus-470-ftir.html <br /> <br /> ==Other links of interest==<br /> [[File:FTIR instrument.png|400px|thumb|left|FTIR instrument]]<br /> [[File:FTIR_cheatsheet_2.gif|400px|thumb|left|FTIR_cheatsheet]]</div>

Kcherry