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Kathryn Saulcy BIOC430 S17

2017-01-21T02:05:12Z

Kate saulcy: Created page with "Chemistry/Biochemistry Research 430 :Spring 2017 :Kate Saulcy :Sophomore Biochemistry Major ==Research Times== Tuesday ~6/7 to 9/10pm : section 01 = 0.25 credit = 4 hours per..."

Chemistry/Biochemistry Research 430
:Spring 2017
:Kate Saulcy
:Sophomore Biochemistry Major

==Research Times==
Tuesday ~6/7 to 9/10pm
: section 01 = 0.25 credit = 4 hours per week.

==Proposed Research Project==
Insulin: It's Structure, Function, and Interaction in Model Cell Membranes

===General Information===
:Advisor: Audra Sostarecz
:Other research student collaborators: none
:Other Research Collaborators: Dr. Debbie C. Crans (Colorado State University)

===Proposal===

:Insulin is a polypeptide hormone that is created and used by a healthy human body to regulate blood sugar. The specific conformation that an insulin molecule adopts affects the stability and functionality of the hormone. Interactions with lipids, specifically Dipalmitoylphosphatidylcholine (DPPC) causes conformational changes in the shape of the insulin molecule and its state of aggregation. The Langmuir technique can be used to create monolayers of insulin, both human recombinant and bovine. Samples of insulin were mixed with lipid, at varying ratios, to determine any molecular interactions between the two compounds and to determine to what extent they interact. At a 25% Insulin/75% DPPC ratio, insulin makes a monolayer of DPPC more fluid. At a 75% Insulin/25% DPPC ratio, DPPC orders the insulin molecules. Subcutaneous injection exposes insulin molecules to lipid and this interaction appears, due to the disappearance of a phase transition on the mixed isotherm, to cause the insulin molecules to adopt a hexameric conformation. As the most active form of insulin is the monomer, this may account for less effective or incorrect dosing of insulin.
In the presence of zinc, insulin is known to bind into hexamers. This is how long-acting medical insulin is stored. The Langmuir technique, with a multi-well plate, can be used to investigate changes in pressure that correspond with changes in molecular area. These changes indicate a conformational change in the insulin molecule and thus a change in effectiveness within the human body.

===Instruments to be used===
:Langmuir monolayer trough

===References (2 minimum)===

http://pubs.acs.org/doi/full/10.1021/la204201w?src=recsys
http://pubs.acs.org/doi/abs/10.1021/jp3046643

(more available upon request)

===Research pledge===
I, Kate Saulcy, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Kathryn Saulcy Chem430 F16

2016-08-28T21:51:20Z

Kate saulcy: /* Proposal */

Biochemistry Research 430
:Fall 2016
:Kate Saulcy
:Sophomore Biochemistry Major

==Research Times==
Wednesday 3-6pm (Trough Lab)

==Proposed Research Project==

===Project Title===
Insulin: Its Structure, Function, and Interaction in Model Cell Membranes

===General Information===
:Advisor: Audra Sostarecz
:Other Research Collaborators: Debbie Crans, Colorado State University

===Proposal===
This semester, I am continuing the research from the Doc Kieft Summer Research Program. My work is based on investigating the effects of insulin, both human and bovine, on various lipids that are common components of biological membranes. I am also working to determine the structure of insulin while altering specific variables: concentration, temperature, and the presence of various metal cations (zinc, copper, etc.). I am using a model membrane system knowing as a Langmuir monolayer to determine the interactions of these substances as though they were in living systems. I will also be using the Atomic Force Microscope to image the various conformations that the insulin molecule may take upon exposure to the metals and other variables previously mentioned.

===Instruments to be used===
:Langmuir Monolayer Trough
:Atomic Force Microscope

===References===
Aggregation of Insulin at the Interface (http://pubs.acs.org/doi/pdf/10.1021/jp4101202?src=recsys)

Surface Chemistry and Spectroscopy of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/full/10.1021/jp3046643)

Study of the Aggregation of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/pdfplus/10.1021/la204201w?src=recsys)

===Research pledge===
I, Kate Saulcy, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Kathryn Saulcy Chem430 F16

2016-08-28T21:50:24Z

Kate saulcy: /* Proposed Research Project */

Biochemistry Research 430
:Fall 2016
:Kate Saulcy
:Sophomore Biochemistry Major

==Research Times==
Wednesday 3-6pm (Trough Lab)

==Proposed Research Project==

===Project Title===
Insulin: Its Structure, Function, and Interaction in Model Cell Membranes

===General Information===
:Advisor: Audra Sostarecz
:Other Research Collaborators: Debbie Crans, Colorado State University

===Proposal===
This semester, I am continuing the research that I did during the Doc Kieft Summer Research Program over the summer. My work is based on investigating the effects of insulin, both human and bovine, on various lipids that are common components of biological membranes. I am also working to determine the structure of insulin while altering specific variables: concentration, temperature, and the presence of various metal cations (zinc, copper, etc.). I am using a model membrane system knowing as a Langmuir monolayer to determine the interactions of these substances as though they were in living systems. I will also be using the Atomic Force Microscope to image the various conformations that the insulin molecule may take upon exposure to the metals and other variables previously mentioned.

===Instruments to be used===
:Langmuir Monolayer Trough
:Atomic Force Microscope

===References===
Aggregation of Insulin at the Interface (http://pubs.acs.org/doi/pdf/10.1021/jp4101202?src=recsys)

Surface Chemistry and Spectroscopy of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/full/10.1021/jp3046643)

Study of the Aggregation of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/pdfplus/10.1021/la204201w?src=recsys)

===Research pledge===
I, Kate Saulcy, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Kathryn Saulcy Chem430 F16

2016-08-28T21:47:14Z

Kate saulcy:

Biochemistry Research 430
:Fall 2016
:Kate Saulcy
:Sophomore Biochemistry Major

==Research Times==
Wednesday 3-6pm (Trough Lab)

==Proposed Research Project==
Studying the structure and determining the function of insulin in the presence of various lipids.

===Project Title===
Insulin: Its Structure, Function, and Interaction in Model Cell Membranes

===General Information===
:Advisor: Audra Sostarecz
:Other Research Collaborators: Debbie Crans, Colorado State University

===Proposal===
This semester, I am continuing the research that I did during the Doc Kieft Summer Research Program over the summer. My work is based on investigating the effects of insulin, both human and bovine, on various lipids that are common components of biological membranes. I am also working to determine the structure of insulin while altering specific variables: concentration, temperature, and the presence of various metal cations (zinc, copper, etc.). I am using a model membrane system knowing as a Langmuir monolayer to determine the interactions of these substances as though they were in living systems. I will also be using the Atomic Force Microscope to image the various conformations that the insulin molecule may take upon exposure to the metals and other variables previously mentioned.

===Instruments to be used===
:Langmuir Monolayer Trough
:Atomic Force Microscope

===References===
Aggregation of Insulin at the Interface (http://pubs.acs.org/doi/pdf/10.1021/jp4101202?src=recsys)

Surface Chemistry and Spectroscopy of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/full/10.1021/jp3046643)

Study of the Aggregation of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/pdfplus/10.1021/la204201w?src=recsys)

===Research pledge===
I, Kate Saulcy, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Kathryn Saulcy Chem430 F16

2016-08-28T21:45:45Z

Kate saulcy: Created page with "Biochemistry Research 430 :Fall 2016 :Kate Saulcy :Sophomore Biochemistry Major ==Research Times== Wednesday 3-6pm (Trough Lab) ==Proposed Research Project== Studying the st..."

Biochemistry Research 430
:Fall 2016
:Kate Saulcy
:Sophomore Biochemistry Major

==Research Times==
Wednesday 3-6pm (Trough Lab)

==Proposed Research Project==
Studying the structure and determining the function of insulin in the presence of various lipids.

===Enter Project Title here===
Insulin: Its Structure, Function, and Interaction in Model Cell Membranes

===General Information===
:Advisor: Audra Sostarecz
:Other Research Collaborators: Debbie Crans, Colorado State University

===Proposal===
This semester, I am continuing the research that I did during the Doc Kieft Summer Research Program over the summer. My work is based on investigating the effects of insulin, both human and bovine, on various lipids that are common components of biological membranes. I am also working to determine the structure of insulin while altering specific variables: concentration, temperature, and the presence of various metal cations (zinc, copper, etc.). I am using a model membrane system knowing as a Langmuir monolayer to determine the interactions of these substances as though they were in living systems. I will also be using the Atomic Force Microscope to image the various conformations that the insulin molecule may take upon exposure to the metals and other variables previously mentioned.

===Instruments to be used===
Langmuir Monolayer Trough
Atomic Force Microscope

===References===
Aggregation of Insulin at the Interface (http://pubs.acs.org/doi/pdf/10.1021/jp4101202?src=recsys)

Surface Chemistry and Spectroscopy of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/full/10.1021/jp3046643)

Study of the Aggregation of Human Insulin Langmuir Monolayer(http://pubs.acs.org/doi/pdfplus/10.1021/la204201w?src=recsys)

===Research pledge===
I, Kate Saulcy, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.