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Jasmine Davila BIOC430 S17

2017-01-20T21:37:40Z

Jda:

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Thursday 2:30 - 6:30

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli is a well known technique.
Recently, Atomic Force Microscopy (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. Using the NTA-His6 system as a platform for the isolated protein and DNA has shown promise of being an efficient way to study desired single-molecule interactions in a stable manner with AFM (2). We thus plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)

Sonicator

Ultra Centrifuge

Nickel Column

SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]

2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Jasmine Davila BIOC430 S17

2017-01-20T21:37:04Z

Jda:

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli is a well known technique.
Recently, Atomic Force Microscopy (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. Using the NTA-His6 system as a platform for the isolated protein and DNA has shown promise of being an efficient way to study desired single-molecule interactions in a stable manner with AFM (2). We thus plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)

Sonicator

Ultra Centrifuge

Nickel Column

SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]

2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Jasmine Davila BIOC430 S17

2017-01-20T21:36:13Z

Jda: /* Research Times */

==Research Times==
Thursday 2:30-6:30

Jasmine Davila Chem430 F16

2016-09-29T02:51:47Z

Jda: /* Proposal */

Jasmine Davila Chem430 F16

2016-09-29T02:40:54Z

Jda: /* Instruments to be used */

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli has been done many times before.
Recently, Atomic Force Microscopy (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. The NTA-His6 system has shown promise of being an efficient and powerful platform for the stable oriented immobilization of proteins in AFM single-molecule studies (2). We plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)

Sonicator

Ultra Centrifuge

Nickel Column

SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]

2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Jasmine Davila Chem430 F16

2016-09-29T02:38:46Z

Jda: /* Research Times */

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli has been done many times before.
Recently, Atomic Force Microscopy (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. The NTA-His6 system has shown promise of being an efficient and powerful platform for the stable oriented immobilization of proteins in AFM single-molecule studies (2). We plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)
Sonicator
Ultra Centrifuge
Nickel Column
SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]

2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Jasmine Davila Chem430 F16

2016-09-29T02:38:25Z

Jda: /* References (2 minimum) */

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12
: section 01

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli has been done many times before.
Recently, Atomic Force Microscopy (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. The NTA-His6 system has shown promise of being an efficient and powerful platform for the stable oriented immobilization of proteins in AFM single-molecule studies (2). We plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)
Sonicator
Ultra Centrifuge
Nickel Column
SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]

2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Jasmine Davila Chem430 F16

2016-09-29T02:38:02Z

Jda: /* Proposal */

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12
: section 01

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli has been done many times before.
Recently, Atomic Force Microscopy (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. The NTA-His6 system has shown promise of being an efficient and powerful platform for the stable oriented immobilization of proteins in AFM single-molecule studies (2). We plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)
Sonicator
Ultra Centrifuge
Nickel Column
SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]
2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Jasmine Davila Chem430 F16

2016-09-29T02:36:28Z

Jda: /* Proposed Research Project */

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12
: section 01

==Proposed Research Project==
===IscR Purification from ''Escherichia coli.'' for AFM Study===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: none
:Other Research Collaborators: none

===Proposal===
Iron sulfur cluster regulator (IscR) is an Fe-S protein that functions as a transcriptional regulator of Fe-S production and other Fe-S protein encoding genes in Escherichia coli (E. coli.). IscR controls the expression of more than 40 genes in E. coli (1). The purification of IscR for studying its many properties in E. coli has been done many times before.
Recently, Atomic Force Microscopy Microscope (AFM) has provided a means of studying receptor ligand interactions that were once not available to us. The NTA-His6 system has shown promise of being an efficient and powerful platform for the stable oriented immobilization of proteins in AFM single-molecule studies (2). We plan to use His-tag sequencing to bind our IscR protein and DNA to a nickel platform and investigate the binding strength of IscR to DNA using AFM techniques.

===Instruments to be used===
AFM (atomic force microscope)
Sonicator
Ultra Centrifuge
Nickel Column
SDS PAGE

===References (2 minimum)===
1 Schwartz CJ, Giel JL, Patschkowski T, Luther C, Ruzicka FJ, Beinert H, Kiley PJ. IscR, an Fe-S cluster-containing transcription factor, represses expression of Escherichia coli genes encoding Fe-S cluster assembly proteins. Proc Natl Acad Sci USA 2001; 98:14895-900 [PubMed: 11742080]
2 Verbelen, C., Gruber, H. J., & Dufrêne, Y. F. (2007). The NTA-His6 bond is strong enough for AFM single-molecular recognition studies.Journal of Molecular Recognition: JMR, 20(6), 490–494. doi:10.1002/jmr.833

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

A Little Bit of Exercise

2016-09-22T22:56:25Z

Jda:

I took a picture of my buddy Khader before and after running up and down the stairs twice with the IR camera. We expected to see a large amount of blood flow to his neck, but when we looked at the thermal images we found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]] [[File:Neck_after_run_overlap.png]] [[File:After_run.png]]

Khader after sprinting up the stairs twice

[[File:Awhile_after.png]]
Khader a few seconds after his sprint

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen from where it was being held before the picture was taken.

A Little Bit of Exercise

2016-09-22T22:54:24Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]] [[File:Neck_after_run_overlap.png]] [[File:After_run.png]]

Khader after sprinting up the stairs twice

[[File:Awhile_after.png]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Awhile after.png

2016-09-22T22:53:47Z

Jda:

A Little Bit of Exercise

2016-09-22T22:52:07Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]] [[File:Neck_after_run_overlap.png]] [[File:After_run.png]]

Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

A Little Bit of Exercise

2016-09-22T22:51:42Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]] [[File:Neck_after_run_overlap.png]] [[File:After_run.png]]
Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

A Little Bit of Exercise

2016-09-22T22:51:16Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]] [[File:Neck_after_run_overlap.png]]
Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]] [[File:After_run.png]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:After run.png

2016-09-22T22:50:50Z

Jda:

A Little Bit of Exercise

2016-09-22T22:49:04Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]] [[File:Neck_after_run_overlap.png]]
Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Neck after run overlap.png

2016-09-22T22:48:38Z

Jda:

File:Neck after run.png

2016-09-22T22:45:28Z

Jda:

A Little Bit of Exercise

2016-09-22T22:43:50Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]] [[File:Khader's_neck.png]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]]
Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Khader's neck.png

2016-09-22T22:43:01Z

Jda:

A Little Bit of Exercise

2016-09-22T22:41:05Z

Jda:

[[File:Petri_dish.JPG]]
[[File:P_dish_overlaped.png]]

A cold petri dish with e. coli and its overlapped thermal image. A thermal thumbprint can be seen.

[[File:Thermal_Khader.JPG]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]]
Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:P dish overlaped.png

2016-09-22T22:38:26Z

Jda: overlapped image of petri dish and its thermal image.

overlapped image of petri dish and its thermal image.

A Little Bit of Exercise

2016-09-22T22:27:47Z

Jda:

[[File:Petri_dish.JPG]]
A cold petri dish.

[[File:Thermal_Khader.JPG]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]]
Khader after sprinting up the stairs twice

[[File:Khader_I_think.JPG]]
Khader a few seconds after his sprint

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Khader I think.JPG

2016-09-22T22:27:01Z

Jda: Khader a few seconds after his sprint

Khader a few seconds after his sprint

A Little Bit of Exercise

2016-09-22T22:25:12Z

Jda:

[[File:Petri_dish.JPG]]
A cold petri dish.

[[File:Thermal_Khader.JPG]]
Khader's neck before the sprint up the stairs

[[File:Khader_thermal_after.JPG]]
Khader after sprinting up the stairs twice

I took a picture of my buddy Khader before and after running up and down the stairs a twice. We expected to see a large amount of blood flow to his neck but found that it all went to his head!

We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Khader thermal after.JPG

2016-09-22T22:23:48Z

Jda: Khader after jogging up the stairs twice

Khader after jogging up the stairs twice

A Little Bit of Exercise

2016-09-22T22:22:15Z

Jda:

[[File:Petri_dish.JPG]]
A cold petri dish.

[[File:Thermal_Khader.JPG]]
Khader's neck before the jog up the stairs

I took a picture of my buddy Khader before and after running up and down the stairs a few times. We expected to see a large amount of blood flow to his neck but found that it all went to his head!
We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Thermal Khader.JPG

2016-09-22T22:20:46Z

Jda: Khader's neck before the jog

Khader's neck before the jog

A Little Bit of Exercise

2016-09-22T22:19:38Z

Jda:

[[File:Petri_dish.JPG]]

I took a picture of my buddy Khader before and after running up and down the stairs a few times. We expected to see a large amount of blood flow to his neck but found that it all went to his head!
We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

File:Petri dish.JPG

2016-09-22T22:16:48Z

Jda: Petri dish from cold room

Petri dish from cold room

A Little Bit of Exercise

2016-09-22T22:15:23Z

Jda: Created page with "I took a picture of my buddy Khader before and after running up and down the stairs a few times. We expected to see a large amount of blood flow to his neck but found that it..."

I took a picture of my buddy Khader before and after running up and down the stairs a few times. We expected to see a large amount of blood flow to his neck but found that it all went to his head!
We also attempted taking pictures of some bacteria being stored in the cold room and found that the petri dish showed no thermal activity. This makes sense since the purpose of storing them there is that they will be preserved in a nonactive state until we take them back out into a warmer environment.

Jasmine Davila Chem430 F16

2016-08-26T21:18:37Z

Jda: Created page with "SChemistry/Biochemistry Research 430 :Fall 2016 :Jasmine Davila :Senior Biochemistry Major ==Research Times== Tuesday 8-12 : section 01 ==Proposed Research Project== ===En..."

SChemistry/Biochemistry Research 430
:Fall 2016
:Jasmine Davila
:Senior Biochemistry Major

==Research Times==
Tuesday 8-12
: section 01

==Proposed Research Project==
===Enter Project Title here===

===General Information===
:Advisor: Laura Moore
:Other research student collaborators: here
:Other Research Collaborators: here

===Proposal===

===Instruments to be used===

===References (2 minimum)===

===Research pledge===
I, Jasmine Davila, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.