http://205.166.159.208/wiki/api.php?action=feedcontributions&feedformat=atom&user=Ian+Salveson 2026-05-03T13:00:13Z User contributions MediaWiki 1.35.5 http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=5118 2017-02-07T01:39:54Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]<br /> <br /> ==July 6, 2016==<br /> <br /> Flash Prep<br /> <br /> [[File:Flash_Prep_070716.jpg|500px]]<br /> <br /> ==July 12, 2016==<br /> <br /> [[File:APAP Stdnmr.JPG|500px]]<br /> <br /> [[File:APAP Std AromaticNMR.JPG|500px]]<br /> <br /> [[File:APAP 1NMR.JPG|500px]]<br /> <br /> ==February 6, 2017==<br /> <br /> Refill 3L of HPLC 0.1%TFA. Check efficacy of recorded APAP oxidation HPLC method. Collect data showing the HPLC signature of the polymeric precipitate. Immobilized HRP.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=5117 2017-02-06T17:12:09Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]<br /> <br /> ==July 6, 2016==<br /> <br /> Flash Prep<br /> <br /> [[File:Flash_Prep_070716.jpg|500px]]<br /> <br /> ==July 12, 2016==<br /> <br /> [[File:APAP Stdnmr.JPG|500px]]<br /> <br /> [[File:APAP Std AromaticNMR.JPG|500px]]<br /> <br /> [[File:APAP 1NMR.JPG|500px]]<br /> <br /> ==February 6, 2017==<br /> <br /> Refill 3L of HPLC 0.1%TFA. Check efficacy of recorded APAP oxidation HPLC method. Collect data showing the HPLC signature of the polymeric precipitate.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson_Chem430_S17&diff=5116 2017-02-06T17:00:20Z

<p>Ian Salveson: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Ian Salveson<br /> :Senior Chemistry Major<br /> <br /> ==Research Times==<br /> Mon: 10AM-12PM + 6-8PM<br /> Meeting Time: Wed. 11:00<br /> <br /> ==Proposed Research Project==<br /> Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP and to investigate the chemical properties of the primary products.<br /> <br /> ===Project Title===<br /> Characterization and Analysis of Acetaminophen Metabolites<br /> <br /> ===General Information===<br /> :Advisor: Dr. Bradley E. Sturgeon<br /> <br /> ===Proposal===<br /> Determine chemical properties of previously isolated primary acetaminophen oxidation products using cyclic voltammetry and ESR spectroscopy. <br /> <br /> ===Instruments to be used===<br /> HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR<br /> ===References (2 minimum)===<br /> 1.) Hinson, Jack A., Dean W. Roberts, and Laura P. James. &quot;Mechanisms of Acetaminophen-Induced Liver Necrosis.&quot; Handbook of Experimental Pharmacology Adverse Drug Reactions (2009): 369-405. Web.<br /> 2.) Potter, David W., Dwight W. Miller, and Jack A. Hinson. &quot;Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase.&quot; Journal of Biological Chemistry 260.22 (1985): 12174-2180. Print.<br /> <br /> <br /> <br /> ===Research pledge===<br /> I, Ian Salveson, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson_Chem430_S17&diff=5099 2017-02-03T19:08:58Z

<p>Ian Salveson: /* Research Times */</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Ian Salveson<br /> :Senior Chemistry Major<br /> <br /> ==Research Times==<br /> Mon: 10AM-12PM + 6-8PM<br /> <br /> ==Proposed Research Project==<br /> Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP and to investigate the chemical properties of the primary products.<br /> <br /> ===Project Title===<br /> Characterization and Analysis of Acetaminophen Metabolites<br /> <br /> ===General Information===<br /> :Advisor: Dr. Bradley E. Sturgeon<br /> <br /> ===Proposal===<br /> Determine chemical properties of previously isolated primary acetaminophen oxidation products using cyclic voltammetry and ESR spectroscopy. <br /> <br /> ===Instruments to be used===<br /> HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR<br /> ===References (2 minimum)===<br /> 1.) Hinson, Jack A., Dean W. Roberts, and Laura P. James. &quot;Mechanisms of Acetaminophen-Induced Liver Necrosis.&quot; Handbook of Experimental Pharmacology Adverse Drug Reactions (2009): 369-405. Web.<br /> 2.) Potter, David W., Dwight W. Miller, and Jack A. Hinson. &quot;Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase.&quot; Journal of Biological Chemistry 260.22 (1985): 12174-2180. Print.<br /> <br /> <br /> <br /> ===Research pledge===<br /> I, Ian Salveson, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson_Chem430_S17&diff=5018 2017-01-31T01:28:12Z

<p>Ian Salveson: Created page with &quot;Chemistry/Biochemistry Research 430 :Spring 2017 :Ian Salveson :Senior Chemistry Major ==Research Times== Mon: 6-8PM ==Proposed Research Project== Investigating the metabol...&quot;</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Spring 2017<br /> :Ian Salveson<br /> :Senior Chemistry Major<br /> <br /> ==Research Times==<br /> Mon: 6-8PM<br /> <br /> <br /> ==Proposed Research Project==<br /> Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP and to investigate the chemical properties of the primary products.<br /> <br /> ===Project Title===<br /> Characterization and Analysis of Acetaminophen Metabolites<br /> <br /> ===General Information===<br /> :Advisor: Dr. Bradley E. Sturgeon<br /> <br /> ===Proposal===<br /> Determine chemical properties of previously isolated primary acetaminophen oxidation products using cyclic voltammetry and ESR spectroscopy. <br /> <br /> ===Instruments to be used===<br /> HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR<br /> ===References (2 minimum)===<br /> 1.) Hinson, Jack A., Dean W. Roberts, and Laura P. James. &quot;Mechanisms of Acetaminophen-Induced Liver Necrosis.&quot; Handbook of Experimental Pharmacology Adverse Drug Reactions (2009): 369-405. Web.<br /> 2.) Potter, David W., Dwight W. Miller, and Jack A. Hinson. &quot;Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase.&quot; Journal of Biological Chemistry 260.22 (1985): 12174-2180. Print.<br /> <br /> <br /> <br /> ===Research pledge===<br /> I, Ian Salveson, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson_Chem430_F16&diff=3595 2016-09-28T14:51:47Z

<p>Ian Salveson: </p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Ian Salveson<br /> :Senior Chemistry Major<br /> <br /> ==Research Times==<br /> M/W/F 10am-12pm<br /> <br /> <br /> ==Proposed Research Project==<br /> Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP.<br /> <br /> ===Project Title===<br /> Characterization and Analysis of Acetaminophen Metabolites<br /> <br /> ===General Information===<br /> :Advisor: Dr. Bradley E. Sturgeon<br /> <br /> ===Proposal===<br /> Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which acetaminophen is oxidized by this type of enzyme.<br /> <br /> ===Instruments to be used===<br /> HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR<br /> ===References (2 minimum)===<br /> 1.) Hinson, Jack A., Dean W. Roberts, and Laura P. James. &quot;Mechanisms of Acetaminophen-Induced Liver Necrosis.&quot; Handbook of Experimental Pharmacology Adverse Drug Reactions (2009): 369-405. Web.<br /> 2.) Potter, David W., Dwight W. Miller, and Jack A. Hinson. &quot;Identification of Acetaminophen Polymerization Products Catalyzed by Horseradish Peroxidase.&quot; Journal of Biological Chemistry 260.22 (1985): 12174-2180. Print.<br /> <br /> <br /> <br /> ===Research pledge===<br /> I, Ian Salveson, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson_Chem430_F16&diff=2968 2016-09-01T04:06:56Z

<p>Ian Salveson: Created page with &quot;Chemistry/Biochemistry Research 430 :Fall 2016 :Ian Salveson :Senior Chemistry Major ==Research Times== M/W/F 10am-12pm ==Proposed Research Project== Investigating the meta...&quot;</p> <hr /> <div>Chemistry/Biochemistry Research 430<br /> :Fall 2016<br /> :Ian Salveson<br /> :Senior Chemistry Major<br /> <br /> ==Research Times==<br /> M/W/F 10am-12pm<br /> <br /> <br /> ==Proposed Research Project==<br /> Investigating the metabolism of acetaminophen modeled by an enzymatic, single-electron oxidation using HRP.<br /> <br /> ===Project Title===<br /> <br /> <br /> ===General Information===<br /> :Advisor: Dr. Bradley E. Sturgeon<br /> <br /> ===Proposal===<br /> Synthesize the primary products of this oxidation and investigate their activity in a similar system to determine the level to which acetaminophen is oxidized by this type of enzyme.<br /> <br /> ===Instruments to be used===<br /> HPLC, Flash Chromotography System, NMR, Immobilized Enzyme column, ESR<br /> ===References (2 minimum)===<br /> 1. <br /> 2. <br /> <br /> <br /> <br /> <br /> ===Research pledge===<br /> I, Ian Salveson, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_C_Salveson&diff=2300 2016-07-21T19:01:32Z

<p>Ian Salveson: /* Presentations */</p> <hr /> <div>&lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> You have reached the personal page of Ian Salveson.<br /> <br /> ==Personal Information==<br /> Senior Chemistry and Classics Major<br /> <br /> Hometown: St. Charles, IL<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Summer 2015: Kieft Summer Research with Brad Sturgeon (Acetaminophen Oxidation)<br /> <br /> Spring 2016: Chem 430 with B. Sturgeon<br /> <br /> Summer 2016: Kieft Summer Research with Brad Sturgeon (Acetaminophen Oxidation)<br /> <br /> ==Presentations==<br /> <br /> June 2014: '''Kieft Summer Research Presentations'''<br /> <br /> Title: A Simulated Study of Acetaminophen Induced Liver Injury'''<br /> <br /> March 2016: '''251th National ACS Meeting''' San Diego, CA <br /> <br /> Poster Title: ''Enzymatic Oxidation of Acetaminophen''<br /> <br /> July 2016: '''Kieft Summer Research Presentations'''<br /> <br /> Title: Oxidation of Biophenols<br /> <br /> ==Interests==<br /> <br /> -Water Polo<br /> -Cooking<br /> -Magic: The Gathering<br /> <br /> ==Career Plans==<br /> <br /> College Chemistry Professor<br /> <br /> ==Projects==<br /> <br /> [[Acetaminophen Oxidation|'''The Mechanism of Acetaminophen Metabolism''']] <br /> <br /> Acetaminophen, also known as paracetamol, is a commonly used over-the-counter pain reliever. It is also present in mixtures of prescribed pain medication. Taken in accordance with dosage suggestions, acetaminophen is processed normally by the body and does not cause any adverse side effects; however, taken in excess, acetaminophen can quickly lead to liver failure and death. Acetaminophen alone is responsible for 50% of transplants due to drug related liver injury. Correct identification of the mechanism of acetaminophen induced liver damage is the first step in treating and preventing acetaminophen related liver failure. The currently accepted mechanism for liver injury due to acetaminophen involves the creation of a highly reactive oxidation product NAPQI. However, research has identified the formation of a radical during the enzymatic oxidation of acetaminophen. This has led to a new hypothesis where radicals are continuously forming and combining into polymeric material that would cause a buildup of insoluble material within the liver, preventing blood flow and leading to necrosis. This research will revolve around investigating this new hypothesis by analyzing the products of controlled enzymatic oxidations of acetaminophen using HPLC alongside with NMR and Mass Spectroscopy for the characterization of these products.<br /> <br /> '''Experiments'''<br /> <br /> Flash Chromatography(2/15/2016)</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_C_Salveson&diff=2291 2016-07-21T18:42:00Z

<p>Ian Salveson: /* Undergraduate Research Activities */</p> <hr /> <div>&lt;!-- ==Personal Information== --&gt;<br /> &lt;!-- ==Undergraduate Research Activities== --&gt;<br /> &lt;!-- ==Presentations== --&gt;<br /> &lt;!-- ==Interests== --&gt;<br /> &lt;!-- ==Career Goals== --&gt;<br /> &lt;!-- --&gt;<br /> &lt;!-- --&gt;<br /> You have reached the personal page of Ian Salveson.<br /> <br /> ==Personal Information==<br /> Senior Chemistry and Classics Major<br /> <br /> Hometown: St. Charles, IL<br /> <br /> ==Undergraduate Research Activities==<br /> <br /> Summer 2015: Kieft Summer Research with Brad Sturgeon (Acetaminophen Oxidation)<br /> <br /> Spring 2016: Chem 430 with B. Sturgeon<br /> <br /> Summer 2016: Kieft Summer Research with Brad Sturgeon (Acetaminophen Oxidation)<br /> <br /> ==Presentations==<br /> <br /> June 2014: '''Kieft Summer Research Presentations'''<br /> <br /> Title: A Simulated Study of Acetaminophen Induced Liver Injury'''<br /> <br /> March 2016: '''251th National ACS Meeting''' San Diego, CA <br /> <br /> Poster Title: ''Enzymatic Oxidation of Acetaminophen''<br /> <br /> ==Interests==<br /> <br /> -Water Polo<br /> -Cooking<br /> -Magic: The Gathering<br /> <br /> ==Career Plans==<br /> <br /> College Chemistry Professor<br /> <br /> ==Projects==<br /> <br /> [[Acetaminophen Oxidation|'''The Mechanism of Acetaminophen Metabolism''']] <br /> <br /> Acetaminophen, also known as paracetamol, is a commonly used over-the-counter pain reliever. It is also present in mixtures of prescribed pain medication. Taken in accordance with dosage suggestions, acetaminophen is processed normally by the body and does not cause any adverse side effects; however, taken in excess, acetaminophen can quickly lead to liver failure and death. Acetaminophen alone is responsible for 50% of transplants due to drug related liver injury. Correct identification of the mechanism of acetaminophen induced liver damage is the first step in treating and preventing acetaminophen related liver failure. The currently accepted mechanism for liver injury due to acetaminophen involves the creation of a highly reactive oxidation product NAPQI. However, research has identified the formation of a radical during the enzymatic oxidation of acetaminophen. This has led to a new hypothesis where radicals are continuously forming and combining into polymeric material that would cause a buildup of insoluble material within the liver, preventing blood flow and leading to necrosis. This research will revolve around investigating this new hypothesis by analyzing the products of controlled enzymatic oxidations of acetaminophen using HPLC alongside with NMR and Mass Spectroscopy for the characterization of these products.<br /> <br /> '''Experiments'''<br /> <br /> Flash Chromatography(2/15/2016)</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2278 2016-07-21T17:02:16Z

<p>Ian Salveson: /* July 12, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]<br /> <br /> ==July 6, 2016==<br /> <br /> Flash Prep<br /> <br /> [[File:Flash_Prep_070716.jpg|500px]]<br /> <br /> ==July 12, 2016==<br /> <br /> [[File:APAP Stdnmr.JPG|500px]]<br /> <br /> [[File:APAP Std AromaticNMR.JPG|500px]]<br /> <br /> [[File:APAP 1NMR.JPG|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2277 2016-07-21T17:01:19Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]<br /> <br /> ==July 6, 2016==<br /> <br /> Flash Prep<br /> <br /> [[File:Flash_Prep_070716.jpg|500px]]<br /> <br /> ==July 12, 2016==<br /> <br /> [[File:APAP Stdnmr.JPG|500px]]<br /> <br /> [[File:APAP Std AromaticNMR.JPG|500px]]<br /> <br /> [[File:APAP 1NMR.JPG.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:APAP_1NMR.JPG&diff=2275 2016-07-21T16:48:32Z

<p>Ian Salveson: NMR of aromatic region of APAP 2-2'Dimer</p> <hr /> <div>NMR of aromatic region of APAP 2-2'Dimer</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:APAP_Std_AromaticNMR.JPG&diff=2274 2016-07-21T16:47:01Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:APAP_Stdnmr.JPG&diff=2273 2016-07-21T16:33:23Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2271 2016-07-21T16:24:03Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]<br /> <br /> ==July 6, 2016==<br /> <br /> Flash Prep<br /> <br /> [[File:Flash_Prep_070716.jpg|500px]]<br /> <br /> ==July 12, 2016==</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Nuclear_Magnetic_Resonance_Spectroscopy&diff=2250 2016-07-13T21:23:31Z

<p>Ian Salveson: Created page with &quot;Nuclear magnetic resonance spectroscopy, also known as NMR spectroscopy, is a tool used for determining physical properties of atoms or their molecules. There are several diff...&quot;</p> <hr /> <div>Nuclear magnetic resonance spectroscopy, also known as NMR spectroscopy, is a tool used for determining physical properties of atoms or their molecules. There are several different types of NMR.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:H_NMR_Cheatsheet.gif&diff=2249 2016-07-13T14:53:43Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2212 2016-07-07T18:25:14Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]<br /> <br /> ==July 6, 2016==<br /> <br /> Flash Prep<br /> <br /> [[File:Flash_Prep_070716.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Flash_Prep_070716.jpg&diff=2211 2016-07-07T18:19:54Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Beaker_Exp_070516.jpg&diff=2202 2016-07-06T15:25:21Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2201 2016-07-06T15:25:10Z

<p>Ian Salveson: /* July 5, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]<br /> <br /> Beaker Experiment<br /> <br /> [[File:Beaker Exp 070516.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Flash_070516.jpg&diff=2197 2016-07-06T15:06:09Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2196 2016-07-06T15:05:34Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]<br /> <br /> ==July 5, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 070516.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2181 2016-06-30T21:18:01Z

<p>Ian Salveson: /* June 29, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fractions<br /> <br /> [[File:Flash 063016.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2180 2016-06-30T21:16:43Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]<br /> <br /> ==June 29, 2016==<br /> <br /> Flash Fraction<br /> <br /> [[File:Flash 063016.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Flash_063016.jpg&diff=2179 2016-06-30T21:12:20Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Beaker_Drip.jpg&diff=2162 2016-06-29T17:56:05Z

<p>Ian Salveson: Ian Salveson uploaded a new version of File:Beaker Drip.jpg</p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2161 2016-06-29T17:54:44Z

<p>Ian Salveson: /* June 28, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==<br /> <br /> Investigated the effects of introducing the peroxide through a drip into a stirred reaction mixture.<br /> <br /> [[File:Beaker Drip.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Beaker_Drip.jpg&diff=2160 2016-06-29T17:48:26Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2159 2016-06-29T17:47:25Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]<br /> <br /> ==June 28, 2016==</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:APAP_062716.jpg&diff=2107 2016-06-28T15:03:49Z

<p>Ian Salveson: Ian Salveson uploaded a new version of File:APAP 062716.jpg</p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2106 2016-06-28T15:03:02Z

<p>Ian Salveson: /* June 27, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2105 2016-06-28T15:02:51Z

<p>Ian Salveson: /* June 27, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.<br /> <br /> [[File:APAP_062716.jpg|400px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:APAP_062716.jpg&diff=2104 2016-06-28T15:01:13Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2103 2016-06-28T14:56:57Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]<br /> <br /> <br /> ==June 27, 2016==<br /> <br /> Compared filtered reaction solution with dioxane solution.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Vial_3_Prep_062316.jpg&diff=2096 2016-06-24T15:08:42Z

<p>Ian Salveson: Ian Salveson uploaded a new version of File:Vial 3 Prep 062316.jpg</p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2095 2016-06-24T15:05:35Z

<p>Ian Salveson: /* June 23, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[File:Vial 3 Prep 062316.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2094 2016-06-24T15:05:07Z

<p>Ian Salveson: /* June 23, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.<br /> <br /> [[Vial 3 Prep 062316.jpg|500px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:Vial_3_Prep_062316.jpg&diff=2093 2016-06-24T15:02:38Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2092 2016-06-24T15:02:13Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.<br /> <br /> ==June 23, 2016==<br /> <br /> Prepared Vial 2 for flash.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2091 2016-06-24T14:54:25Z

<p>Ian Salveson: /* June 22, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]<br /> <br /> There appear to be two impurities which are unaccounted for in the complete mixture.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2090 2016-06-24T14:45:52Z

<p>Ian Salveson: /* June 16, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|500px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|500px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2089 2016-06-24T14:45:34Z

<p>Ian Salveson: /* June 20, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|500px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2088 2016-06-24T14:45:20Z

<p>Ian Salveson: /* June 22, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|400px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|500px|]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2087 2016-06-24T14:45:05Z

<p>Ian Salveson: /* June 22, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|400px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak. Below is a chromatogram of the complete mixture shown with the peak collected through flash.<br /> <br /> [[File:APAP Flash 062216.jpg|400px|]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=File:APAP_Flash_062216.jpg&diff=2086 2016-06-24T14:42:44Z

<p>Ian Salveson: </p> <hr /> <div></div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2085 2016-06-24T14:42:00Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|400px]]<br /> <br /> ==June 22, 2016==<br /> <br /> Peformed flash Chromotography on the prepped APAP sample from 6/20. Was able to collect a nonstandard peak.</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2069 2016-06-22T14:47:32Z

<p>Ian Salveson: /* June 20, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> <br /> [[File:Prepped-APAP062016.jpg|400px]]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2068 2016-06-22T14:47:05Z

<p>Ian Salveson: </p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample C from June 16th was Lyophilized and reconstituted in a solution of 90% water and 10% ACN. This solution was filtered and analyzed once more using the HPLC see if any product was lost.<br /> [[File:Prepped-APAP062016.jpg|400px]</div>

Ian Salveson http://205.166.159.208/wiki/index.php?title=Ian_Salveson:_Research_Log&diff=2067 2016-06-22T14:43:40Z

<p>Ian Salveson: /* June 20, 2016 */</p> <hr /> <div>==June 15, 2016==<br /> Determine the effect of flow rate on product formation. Figure 1 below shows similar reactions with differing flow rates.<br /> <br /> [[File:APAP-2mM-IE-Flow-Rate.jpg|500px|Figure 1]]<br /> <br /> This figure shows flow rate has a small effect on product formation. This experiment was done at 2 additional concentrations with similar results.<br /> <br /> ==June 16, 2016==<br /> <br /> Concentration of acetaminophen is increased in preparation for Flash Chromatography. Figure 1 below shows the effect of increasing concentration on product formation.<br /> <br /> [[File:APAP-IE-50mM-1ml-min.jpg|400px|Figure 1]]<br /> <br /> It appears that at higher concentrations, the reaction will begin to form polymeric material, despite relatively low concentrations of Hydrogen Peroxide.<br /> <br /> Sample B will be separated using Flash Chromatography.<br /> <br /> It is important to note that we have reached the max absorbance of our instrument.<br /> <br /> Figure 2 below shows a set of samples under identical conditions as Figure 1 except with a flow rate of 2 ml/min.<br /> <br /> [[File:APAP-IE-50mM-2ml-min.jpg|400px|Figure 2]]<br /> <br /> The chromatography on this figure is faulty, specifically the changing retention time. The results also contradict what may have been expected of a faster flow rate. These samples should be run again if the data is deemed significant.<br /> <br /> ==June 20, 2016==<br /> Sample</div>

Ian Salveson