MC Chem Wiki - User contributions [en]

Chlorogenic acid isolation

2017-02-20T21:52:40Z

DFX: /* Current Progress */

==Introduction==
Chlorogenic acid ([[CGA|CGA]]) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.

[[File:800px-Chlorogenic-acid-from-CAS-2D-skeletal.png|240px]]

Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big

==Proposed Project Description==

Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

'''References'''

1):[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]

2):[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

==Current Progress==

'''Sept 2016'''

'''9/20'''
[[Extraction_Method|Extraction method]]

'''9/22'''
Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a [[CGA_TLC|TLC]] plate with a CGA standard on it. Method works!

'''9/27'''
Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more [[CGA_TLC|TLC]] was performed with thee two 35 mesh samples.

'''9/28'''
First use the UV-Vis, to find a spectra of the sample, finding a maximum at about 330. Then ran a flash column of with a silica column with ethyl acetate and hexanes as the mobile phase nothing flowed off of the column after thirty minutes of run time. After flushing the column with water it flow off of the column. The ran a [[CGA_TLC|TLC]] with the sample to compare the eluding power of ethyl acetate to methanol.

'''9/29'''
Ran another flash column with a C18 column, with acidified water and acetonitrile as mobile phases. The first run had sample flow out in the very beginning of the run at 100% water, the second run started at 50% water 50% acetonitrile and again it ran off very early, the third and final attempt started with 100% acetonitrile and still it flowed off the column in the very beginning of the run. While running the samples the sample was filtered to leave the green bean particles behind and then 15ml was placed in a vial in a freezer to be lyophilized.

'''Feb 2017'''

'''2/1'''
Purification method - After the crude is extracted it is roto-vaped to evaporate the methanol, and washed twice with chloroform to pull out the caffeine and wax and washed again with Pet-Ether two more times to pull out the lipids and pigments

'''2/7'''
[[CGA_HPLC|HPLC]] data was collected and examined

'''2/13'''
[[CGA_NMR|NMR]] Trials are being done for the rest of this week

Chlorogenic acid isolation

2017-02-20T18:15:54Z

DFX: /* Current Progress */

==Introduction==
Chlorogenic acid ([[CGA|CGA]]) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.

[[File:800px-Chlorogenic-acid-from-CAS-2D-skeletal.png|240px]]

Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big

==Proposed Project Description==

Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

'''References'''

1):[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]

2):[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

==Current Progress==

'''Sept 2016'''

'''9/20'''
[[Extraction_Method|Extraction method]]

'''9/22'''
Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a [[CGA_TLC|TLC]] plate with a CGA standard on it. Method works!

'''9/27'''
Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more [[CGA_TLC|TLC]] was performed with thee two 35 mesh samples.

'''9/28'''
First use the UV-Vis, to find a spectra of the sample, finding a maximum at about 330. Then ran a flash column of with a silica column with ethyl acetate and hexanes as the mobile phase nothing flowed off of the column after thirty minutes of run time. After flushing the column with water it flow off of the column. The ran a [[CGA_TLC|TLC]] with the sample to compare the eluding power of ethyl acetate to methanol.

'''9/29'''
Ran another flash column with a C18 column, with acidified water and acetonitrile as mobile phases. The first run had sample flow out in the very beginning of the run at 100% water, the second run started at 50% water 50% acetonitrile and again it ran off very early, the third and final attempt started with 100% acetonitrile and still it flowed off the column in the very beginning of the run. While running the samples the sample was filtered to leave the green bean particles behind and then 15ml was placed in a vial in a freezer to be lyophilized.

'''Feb 2017'''

'''2/1'''
Purification method - After the crude is extracted it is roto-vaped to evaporate the methanol, and washed twice with chloroform to pull out the pigments and washed again with Pet-Ether two more times to pull out the fats and lipids.

'''2/7'''
[[CGA_HPLC|HPLC]] data was collected and examined

'''2/13'''
[[CGA_NMR|NMR]] Trials are being done for the rest of this week

Chlorogenic acid isolation

2017-02-20T18:15:37Z

DFX: /* Current Progress */

==Introduction==
Chlorogenic acid ([[CGA|CGA]]) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.

[[File:800px-Chlorogenic-acid-from-CAS-2D-skeletal.png|240px]]

Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big

==Proposed Project Description==

Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

'''References'''

1):[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]

2):[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

==Current Progress==

'''Sept 2016'''

'''9/20'''
[[Extraction_Method|Extraction method]]

'''9/22'''
Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a [[CGA_TLC|TLC]] plate with a CGA standard on it. Method works!

'''9/27'''
Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more [[CGA_TLC|TLC]] was performed with thee two 35 mesh samples.

'''9/28'''
First use the UV-Vis, to find a spectra of the sample, finding a maximum at about 330. Then ran a flash column of with a silica column with ethyl acetate and hexanes as the mobile phase nothing flowed off of the column after thirty minutes of run time. After flushing the column with water it flow off of the column. The ran a [[CGA_TLC|TLC]] with the sample to compare the eluding power of ethyl acetate to methanol.

'''9/29'''
Ran another flash column with a C18 column, with acidified water and acetonitrile as mobile phases. The first run had sample flow out in the very beginning of the run at 100% water, the second run started at 50% water 50% acetonitrile and again it ran off very early, the third and final attempt started with 100% acetonitrile and still it flowed off the column in the very beginning of the run. While running the samples the sample was filtered to leave the green bean particles behind and then 15ml was placed in a vial in a freezer to be lyophilized.

'''Feb 2017'''

'''2/1'''
Purification method --> After the crude is extracted it is roto-vaped to evaporate the methanol, and washed twice with chloroform to pull out the pigments and washed again with Pet-Ether two more times to pull out the fats and lipids.

'''2/7'''
[[CGA_HPLC|HPLC]] data was collected and examined

'''2/13'''
[[CGA_NMR|NMR]] Trials are being done for the rest of this week

CGA NMR

2017-02-20T18:07:58Z

DFX: /* Simulated NMR */

==Simulated NMR==
[[File:CGA Spectra.jpg|720px|thumb|left|The NMR that is to be expected when the standard spectra it taken]]

File:CGA Spectra.jpg

2017-02-20T18:06:10Z

DFX:

CGA NMR

2017-02-20T18:05:36Z

DFX: Created page with "==Simulated NMR=="

==Simulated NMR==

Chlorogenic acid isolation

2017-02-20T18:05:09Z

DFX: /* Current Progress */

==Introduction==
Chlorogenic acid ([[CGA|CGA]]) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.

[[File:800px-Chlorogenic-acid-from-CAS-2D-skeletal.png|240px]]

Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big

==Proposed Project Description==

Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

'''References'''

1):[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]

2):[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

==Current Progress==

'''Sept 2016'''

'''9/20'''
[[Extraction_Method|Extraction method]]

'''9/22'''
Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a [[CGA_TLC|TLC]] plate with a CGA standard on it. Method works!

'''9/27'''
Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more [[CGA_TLC|TLC]] was performed with thee two 35 mesh samples.

'''9/28'''
First use the UV-Vis, to find a spectra of the sample, finding a maximum at about 330. Then ran a flash column of with a silica column with ethyl acetate and hexanes as the mobile phase nothing flowed off of the column after thirty minutes of run time. After flushing the column with water it flow off of the column. The ran a [[CGA_TLC|TLC]] with the sample to compare the eluding power of ethyl acetate to methanol.

'''9/29'''
Ran another flash column with a C18 column, with acidified water and acetonitrile as mobile phases. The first run had sample flow out in the very beginning of the run at 100% water, the second run started at 50% water 50% acetonitrile and again it ran off very early, the third and final attempt started with 100% acetonitrile and still it flowed off the column in the very beginning of the run. While running the samples the sample was filtered to leave the green bean particles behind and then 15ml was placed in a vial in a freezer to be lyophilized.

'''Feb 2017'''

'''2/7'''
[[CGA_HPLC|HPLC]] data was collected and examined

'''2/13'''
[[CGA_NMR|NMR]] Trials are being done for the rest of this week

Particle in a Box

2017-02-09T23:25:02Z

DFX:

'''Question: Can a simple function like y(x) = Sqrt[a/2]*Sin[Pi*x/a] describe an electron in a molecule?'''

'''Answer: yes.'''

===The Basic Model System===
1) Consider a box with no lid with infinitely high walls. This box represents the "molecular frame" for which the electron can exist.

2) When a particle/electron is placed inside this box, it has a particular "energy." Considering that "energy" is a ''measurable quantity'' in systems described by classical mechanics, Q.M. postulate 2 tells us that there exists a corresponding operator that can be used to predict the energy or as we say this ''observable''.

3) As with all "operator algebra" problems, there are 3 parts:
:- the operator
:- the (eigen)function
:- the eigenvalue = observable.

The operator in this case, like many, is the ''Hamiltonian'' operator, the eigenfunction is given above, and we need to solve for the eigenvalue or energy.

===Initial Tasks===
1) Convince yourself that the function provided above is an eigenfunction of the Hamiltonian operator.
:You ask, how do i do that?...just operate on the function with the Hamiltonian operator and if you return a "value" and the function, then the function is an eigenfunction for the Hamiltonian operator. You do need to know that the potential energy, V(x), inside of the box and it is equal to zero...so the Hamiltonian operator is a bit more simple in this model system. '''Please "do this" work in your lab notebook.

2) Notice that although this function is an eigenfunction of the Hamiltonian operator, there may be other solutions...any ideas?
:Please plot this function in Mathematica..
:Note: Boundary conditions y(0) = 0 and y(a) = 0
:See figure 4.2/4.3 Reproduce these in Mathematica
:why are these offset as they are?

3) Use the "Boltzmann" distribution to calculate the number of particles in the n=1 verses the n=2 state...essentially reproduce graph in example problem 2.1.

===The Chemical System===
Pi electrons in conjugated molecules can be treated as "particles in a box." See section 5.3.
:1,4-diphenyl-1,3-butadiene (4 pi-electrons)
:1,6-diphenyl-1,3,5-hexatriene (6 pi-electrons)
:1,8-diphenyl-1,3,5,7-octatetraene (8 pi-electrons)

1) What is an estimate of the "box length" (ie molecular frame).

2) What is the DeltaE between the HOMO and LUMO

3) Use the "Boltzmann" distribution to calculate the number of particles in the n(HOMO) verses the n(LUMO) state...essentially reproduce graph in example problem 2.1.

===Data Collection===
1) collect UV-Vis data for a conjugated dye.

2) record structure in lab notebook.

3) can you predict the box length based on lambda max?

===Results===
[[Pinacyanol_chloride|Pinacyanol chloride]]

[[Cryptocyanine|Cryptocyanine]]

[[3,3′Diethylthiacarbocyanine iodide|3,3′Diethylthiacarbocyanine iodide_AK]]

[[3,3'-Diethylthiacarbocyanine iodide|3,3'-Diethylthiacarbocyanine iodide_MA]]

[[3,3'-Diethylthiacarbocyanine iodide CF|3,3'-Diethylthiacarbocyanine iodide_CF]]

Particle in a Box

2017-02-09T23:24:16Z

DFX:

'''Question: Can a simple function like y(x) = Sqrt[a/2]*Sin[Pi*x/a] describe an electron in a molecule?'''

'''Answer: yes.'''

===The Basic Model System===
1) Consider a box with no lid with infinitely high walls. This box represents the "molecular frame" for which the electron can exist.

2) When a particle/electron is placed inside this box, it has a particular "energy." Considering that "energy" is a ''measurable quantity'' in systems described by classical mechanics, Q.M. postulate 2 tells us that there exists a corresponding operator that can be used to predict the energy or as we say this ''observable''.

3) As with all "operator algebra" problems, there are 3 parts:
:- the operator
:- the (eigen)function
:- the eigenvalue = observable.

The operator in this case, like many, is the ''Hamiltonian'' operator, the eigenfunction is given above, and we need to solve for the eigenvalue or energy.

===Initial Tasks===
1) Convince yourself that the function provided above is an eigenfunction of the Hamiltonian operator.
:You ask, how do i do that?...just operate on the function with the Hamiltonian operator and if you return a "value" and the function, then the function is an eigenfunction for the Hamiltonian operator. You do need to know that the potential energy, V(x), inside of the box and it is equal to zero...so the Hamiltonian operator is a bit more simple in this model system. '''Please "do this" work in your lab notebook.

2) Notice that although this function is an eigenfunction of the Hamiltonian operator, there may be other solutions...any ideas?
:Please plot this function in Mathematica..
:Note: Boundary conditions y(0) = 0 and y(a) = 0
:See figure 4.2/4.3 Reproduce these in Mathematica
:why are these offset as they are?

3) Use the "Boltzmann" distribution to calculate the number of particles in the n=1 verses the n=2 state...essentially reproduce graph in example problem 2.1.

===The Chemical System===
Pi electrons in conjugated molecules can be treated as "particles in a box." See section 5.3.
:1,4-diphenyl-1,3-butadiene (4 pi-electrons)
:1,6-diphenyl-1,3,5-hexatriene (6 pi-electrons)
:1,8-diphenyl-1,3,5,7-octatetraene (8 pi-electrons)

1) What is an estimate of the "box length" (ie molecular frame).

2) What is the DeltaE between the HOMO and LUMO

3) Use the "Boltzmann" distribution to calculate the number of particles in the n(HOMO) verses the n(LUMO) state...essentially reproduce graph in example problem 2.1.

===Data Collection===
1) collect UV-Vis data for a conjugated dye.

2) record structure in lab notebook.

3) can you predict the box length based on lambda max?

===Results===
[[Pinacyanol_chloride|Pinacyanol chloride]]

[[Cryptocyanine|Cryptocyanine]]

[[3,3′Diethylthiacarbocyanine iodide_AK|3,3′Diethylthiacarbocyanine iodide]]

[[3,3'-Diethylthiacarbocyanine iodide_MA|3,3'-Diethylthiacarbocyanine iodide]]

[[3,3'-Diethylthiacarbocyanine iodide_CF|3,3'-Diethylthiacarbocyanine iodide CF]]

Particle in a Box

2017-02-09T23:21:01Z

DFX: /* Results */

'''Question: Can a simple function like y(x) = Sqrt[a/2]*Sin[Pi*x/a] describe an electron in a molecule?'''

'''Answer: yes.'''

===The Basic Model System===
1) Consider a box with no lid with infinitely high walls. This box represents the "molecular frame" for which the electron can exist.

2) When a particle/electron is placed inside this box, it has a particular "energy." Considering that "energy" is a ''measurable quantity'' in systems described by classical mechanics, Q.M. postulate 2 tells us that there exists a corresponding operator that can be used to predict the energy or as we say this ''observable''.

3) As with all "operator algebra" problems, there are 3 parts:
:- the operator
:- the (eigen)function
:- the eigenvalue = observable.

The operator in this case, like many, is the ''Hamiltonian'' operator, the eigenfunction is given above, and we need to solve for the eigenvalue or energy.

===Initial Tasks===
1) Convince yourself that the function provided above is an eigenfunction of the Hamiltonian operator.
:You ask, how do i do that?...just operate on the function with the Hamiltonian operator and if you return a "value" and the function, then the function is an eigenfunction for the Hamiltonian operator. You do need to know that the potential energy, V(x), inside of the box and it is equal to zero...so the Hamiltonian operator is a bit more simple in this model system. '''Please "do this" work in your lab notebook.

2) Notice that although this function is an eigenfunction of the Hamiltonian operator, there may be other solutions...any ideas?
:Please plot this function in Mathematica..
:Note: Boundary conditions y(0) = 0 and y(a) = 0
:See figure 4.2/4.3 Reproduce these in Mathematica
:why are these offset as they are?

3) Use the "Boltzmann" distribution to calculate the number of particles in the n=1 verses the n=2 state...essentially reproduce graph in example problem 2.1.

===The Chemical System===
Pi electrons in conjugated molecules can be treated as "particles in a box." See section 5.3.
:1,4-diphenyl-1,3-butadiene (4 pi-electrons)
:1,6-diphenyl-1,3,5-hexatriene (6 pi-electrons)
:1,8-diphenyl-1,3,5,7-octatetraene (8 pi-electrons)

1) What is an estimate of the "box length" (ie molecular frame).

2) What is the DeltaE between the HOMO and LUMO

3) Use the "Boltzmann" distribution to calculate the number of particles in the n(HOMO) verses the n(LUMO) state...essentially reproduce graph in example problem 2.1.

===Data Collection===
1) collect UV-Vis data for a conjugated dye.

2) record structure in lab notebook.

3) can you predict the box length based on lambda max?

===Results===
[[Pinacyanol_chloride|Pinacyanol chloride]]

[[Cryptocyanine|Cryptocyanine]]

[[3,3′Diethylthiacarbocyanine iodide|3,3′Diethylthiacarbocyanine iodide]]

[[3,3'-Diethylthiacarbocyanine iodide|3,3'-Diethylthiacarbocyanine iodide]]

[[3,3'-Diethylthiacarbocyanine iodide CF|3,3'-Diethylthiacarbocyanine iodide CF]]

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:14:54Z

DFX:

==Info==
AKA: 3-Ethyl-2-(3-[3-ethyl-2(3H)benzothiazolylidene]-1-propenyl)benzothiazolium iodide

Empirical Formula: C21H21IN2S2

Molecular Weight: 492.44

MP: 268-270 °C

Absorption: λmax 560 nm

Referance: http://www.sigmaaldrich.com/catalog/product/aldrich/173738?lang=en&region=US
==Graphics==

[[File:3,3′-Diethylthiacarbocyanine iodide.png|720px|thumb|left|The structure of 3,3′-Diethylthiacarbocyanine iodide]]

[[File:3,3'-Diethylthiacarbocyanine iodide.jpg|720px|thumb|left|A spectrum of 3,3′Diethylthiacarbocyanine iodide]]

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:14:42Z

DFX:

==Info==
AKA: 3-Ethyl-2-(3-[3-ethyl-2(3H)benzothiazolylidene]-1-propenyl)benzothiazolium iodide
Empirical Formula: C21H21IN2S2

Molecular Weight: 492.44

MP: 268-270 °C

Absorption: λmax 560 nm

Referance: http://www.sigmaaldrich.com/catalog/product/aldrich/173738?lang=en&region=US
==Graphics==

[[File:3,3′-Diethylthiacarbocyanine iodide.png|720px|thumb|left|The structure of 3,3′-Diethylthiacarbocyanine iodide]]

[[File:3,3'-Diethylthiacarbocyanine iodide.jpg|720px|thumb|left|A spectrum of 3,3′Diethylthiacarbocyanine iodide]]

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:14:20Z

DFX:

==Info==
AKA: 3-Ethyl-2-(3-[3-ethyl-2(3H)benzothiazolylidene]-1-propenyl)benzothiazolium iodide
Empirical Formula: C21H21IN2S2
Molecular Weight: 492.44
mp: 268-270 °C
absorption: λmax 560 nm

Referance: http://www.sigmaaldrich.com/catalog/product/aldrich/173738?lang=en&region=US
==Graphics==

[[File:3,3′-Diethylthiacarbocyanine iodide.png|720px|thumb|left|The structure of 3,3′-Diethylthiacarbocyanine iodide]]

[[File:3,3'-Diethylthiacarbocyanine iodide.jpg|720px|thumb|left|A spectrum of 3,3′Diethylthiacarbocyanine iodide]]

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:10:34Z

DFX: /* 3,3′-Diethylthiacarbocyanine iodide */

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:09:30Z

DFX:

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:09:13Z

DFX:

3,3′Diethylthiacarbocyanine iodide

2017-02-09T23:08:42Z

DFX: Created page with "==3,3′-Diethylthiacarbocyanine iodide== The structure of 3,3′-Diethylthiacarbocyanine iodide File:3,..."

Particle in a Box

2017-02-09T23:06:09Z

DFX: /* Results */

Particle in a Box

2017-02-09T23:05:53Z

DFX:

'''Question: Can a simple function like y(x) = Sqrt[a/2]*Sin[Pi*x/a] describe an electron in a molecule?'''

'''Answer: yes.'''

===The Basic Model System===
1) Consider a box with no lid with infinitely high walls. This box represents the "molecular frame" for which the electron can exist.

2) When a particle/electron is placed inside this box, it has a particular "energy." Considering that "energy" is a ''measurable quantity'' in systems described by classical mechanics, Q.M. postulate 2 tells us that there exists a corresponding operator that can be used to predict the energy or as we say this ''observable''.

3) As with all "operator algebra" problems, there are 3 parts:
:- the operator
:- the (eigen)function
:- the eigenvalue = observable.

The operator in this case, like many, is the ''Hamiltonian'' operator, the eigenfunction is given above, and we need to solve for the eigenvalue or energy.

===Initial Tasks===
1) Convince yourself that the function provided above is an eigenfunction of the Hamiltonian operator.
:You ask, how do i do that?...just operate on the function with the Hamiltonian operator and if you return a "value" and the function, then the function is an eigenfunction for the Hamiltonian operator. You do need to know that the potential energy, V(x), inside of the box and it is equal to zero...so the Hamiltonian operator is a bit more simple in this model system. '''Please "do this" work in your lab notebook.

2) Notice that although this function is an eigenfunction of the Hamiltonian operator, there may be other solutions...any ideas?
:Please plot this function in Mathematica..
:Note: Boundary conditions y(0) = 0 and y(a) = 0
:See figure 4.2/4.3 Reproduce these in Mathematica
:why are these offset as they are?

3) Use the "Boltzmann" distribution to calculate the number of particles in the n=1 verses the n=2 state...essentially reproduce graph in example problem 2.1.

===The Chemical System===
Pi electrons in conjugated molecules can be treated as "particles in a box." See section 5.3.
:1,4-diphenyl-1,3-butadiene (4 pi-electrons)
:1,6-diphenyl-1,3,5-hexatriene (6 pi-electrons)
:1,8-diphenyl-1,3,5,7-octatetraene (8 pi-electrons)

1) What is an estimate of the "box length" (ie molecular frame).

2) What is the DeltaE between the HOMO and LUMO

3) Use the "Boltzmann" distribution to calculate the number of particles in the n(HOMO) verses the n(LUMO) state...essentially reproduce graph in example problem 2.1.

===Data Collection===
1) collect UV-Vis data for a conjugated dye.

2) record structure in lab notebook.

3) can you predict the box length based on lambda max?

===Results===
[[Pinacyanol_chloride|Pinacyanol chloride]]

[[Cryptocyanine|Cryptocyanine]]

[[3,3′-Diethylthiacarbocyanine iodide|3,3′Diethylthiacarbocyanine iodide]]

File:3,3′-Diethylthiacarbocyanine iodide.png

2017-02-09T23:05:04Z

DFX:

File:3,3'-Diethylthiacarbocyanine iodide.jpg

2017-02-09T23:04:48Z

DFX: DFX uploaded a new version of File:3,3'-Diethylthiacarbocyanine iodide.jpg

File:3,3'-Diethylthiacarbocyanine iodide.jpg

2017-02-09T23:04:34Z

DFX: DFX uploaded a new version of File:3,3'-Diethylthiacarbocyanine iodide.jpg

File:3,3'-Diethylthiacarbocyanine iodide.jpg

2017-02-09T23:04:19Z

DFX: DFX uploaded a new version of File:3,3'-Diethylthiacarbocyanine iodide.jpg

File:3,3'-Diethylthiacarbocyanine iodide.jpg

2017-02-09T23:03:44Z

DFX:

Alan Kuhlemier

2017-02-07T22:00:40Z

DFX: /* Undergraduate Research Activities */

==Personal Information==
Senior Chemistry Major

Hometown: Erie IL

Contact Info: 309-507-0967

==Undergraduate Research Activities==

Fall 2016: Isolation of chlorogenic acid from green coffee beans with Brad Sturgeon

Fall 2016: [[Chlorogenic_acid_isolation‎|Chlorogenic acid Isolation‎]]

Spring 2017: [[Chlorogenic_acid_isolation‎|Chlorogenic acid Isolation‎]]

==Presentations==

==Interests==
Gaming, Movies, and the NFL

==Career Plans==
Working for Sigma Aldrich

CGA HPLC

2017-02-07T21:58:12Z

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File:Standard+CHCL3.PNG

2017-02-07T21:57:55Z

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File:Standard.PNG

2017-02-07T21:57:37Z

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File:Standard+NH3SO4.PNG

2017-02-07T21:54:10Z

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CGA HPLC

2017-02-07T21:52:18Z

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==HPLC DATA==

[[File:Standard+PETether.PNG|480px|thumb|left|The standard compared to the sample after rinsing with Chloroform and Pet-ether]]

CGA HPLC

2017-02-07T21:52:07Z

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==HPLC DATA==

[[File:Standard+PETether.PNG|720px|thumb|left|The standard compared to the sample after rinsing with Chloroform and Pet-ether]]

File:Standard+PETether.PNG

2017-02-07T21:50:19Z

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CGA HPLC

2017-02-07T21:48:16Z

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==HPLC DATA==

Standard.pdf

CGA HPLC

2017-02-07T21:46:10Z

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==HPLC DATA==

[[File:Standard.pdf |720px|thumb|left|A CGA standard]]

File:Std+petether.pdf

2017-02-07T21:44:13Z

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File:Std+NH3SO4.pdf

2017-02-07T21:43:55Z

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File:Std+CHcl3.pdf

2017-02-07T21:43:37Z

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File:Standard.pdf

2017-02-07T21:42:59Z

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Chlorogenic acid isolation

2017-02-07T21:42:23Z

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==Introduction==
Chlorogenic acid ([[CGA|CGA]]) has been said to have beneficial biological properties such as lowering blood pressure and a laxative effect. CGA can be found in several plant like prunes, eggplants, peaches, bamboo and green coffee beans. CGA has been trademarked as Svetol, and is added to many food products like chewing gum and mints.

[[File:800px-Chlorogenic-acid-from-CAS-2D-skeletal.png|240px]]

Chlorogenic acids are being used in health drinks and in high demand, finding a better way to extract the CGA would be big

==Proposed Project Description==

Extraction chlorogenic acid from green coffee beans. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

'''References'''

1):[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]

2):[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

==Current Progress==

'''Sept 2016'''

'''9/20'''
[[Extraction_Method|Extraction method]]

'''9/22'''
Ground green coffee beans with a blade grinder. Gather the 35 mesh and 60 mesh sized particles. Made 500 mL of 70/30 methanol/water. Shook two 50 mL tubes of .5 g of the green coffee bean particles and 50 mL methanol/water for 1 hour. After shaking let the green coffee bean particles settle, then ran a [[CGA_TLC|TLC]] plate with a CGA standard on it. Method works!

'''9/27'''
Shook 1 tube of the 35 mesh for two hours to see if time shaken effects the amount of CGA extracted. 1 more [[CGA_TLC|TLC]] was performed with thee two 35 mesh samples.

'''9/28'''
First use the UV-Vis, to find a spectra of the sample, finding a maximum at about 330. Then ran a flash column of with a silica column with ethyl acetate and hexanes as the mobile phase nothing flowed off of the column after thirty minutes of run time. After flushing the column with water it flow off of the column. The ran a [[CGA_TLC|TLC]] with the sample to compare the eluding power of ethyl acetate to methanol.

'''9/29'''
Ran another flash column with a C18 column, with acidified water and acetonitrile as mobile phases. The first run had sample flow out in the very beginning of the run at 100% water, the second run started at 50% water 50% acetonitrile and again it ran off very early, the third and final attempt started with 100% acetonitrile and still it flowed off the column in the very beginning of the run. While running the samples the sample was filtered to leave the green bean particles behind and then 15ml was placed in a vial in a freezer to be lyophilized.

'''Feb 2017'''

'''2/7'''
[[CGA_HPLC|HPLC]] data was collected and examined

Alan Kuhlemier Chem430 S17

2017-01-30T19:27:51Z

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Chemistry Research 430
:Spring 2017
:Alan Kuhlemier
:Senior Chemistry Major

==Research Times==
:M 3-5
:T 3-5
:W 3-7
:section 02 = 0.50 credit = 8 hours per week.

==Proposed Research Project==
===Isolation of Chlorogenic Acid from green coffee beans===
===General Information===
:Advisor: Brad Sturgeon

===Proposal===

[[Chlorogenic_acid_isolation‎|Chlorogenic acid Isolation‎]] from green coffee beans using a [[Ball_mill|ball mill]], and a methanol/water mixture. Purification of the crude extract with flash chromatography, and refining the process improving upon it.

===Instruments to be used===
CombiFlash RF 200i

===References (2 minimum)===
References
:[[Media:Jf9605254.pdf|Five ways to Isolate Chlorogenic acid.]]
:[[Media:Isolation microwave.pdf|Microwave assisted extraction with other references to standard solvent extractions.]]

===Research pledge===
I, Alan Kuhlemier, have read the Chem/Bioc 430 course syllabus and understand the general structure and expectations of the research program. The above material was prepared after consultation, and in conjunction with my research advisor.

Laser Cutter Emission Spectra

2017-01-26T23:55:28Z

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==Intro==
The laser cutter emits a bright white light while cutting, this emission spectra was taken in search of a reason for the white light. The data was collected while vector cutting a circle in to a piece of wood and again with the photo-diode array detector placed in the cutting area while the laser was active to get a better angle to catch the laser in the tube as it was being sent to the cutting head.

==Graph==
[[File:LaserGraph.jpg|720px|thumb|left|Emission spectra of the laser cutter in use]]

Laser Cutter Emission Spectra

2017-01-26T23:54:16Z

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==Intro==
The laser cutter emits a bright white light while cutting, this emission spectra was taken in search of a reason for the white light. The data was collected while vector cutting a circle in to a piece of wood, while the photo-diode array detector was placed in the cutting area while the laser was active to get a better angle to catch the laser in the tube as it was being sent to the cutting head.

==Graph==
[[File:LaserGraph.jpg|720px|thumb|left|Emission spectra of the laser cutter in use]]

Light lab Pchem

2017-01-26T23:53:33Z

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Welcome to the Light lab.

In the introductory chapter we were exposed to a series of experiments that were not appropriately described by the current theory, ie. classical mechanics. These experiments lead to the development of quantum mechanics. Many of these experiments involved "light." In this lab activity we will explore light at a deeper level.

==Examples==
[[IPhone_Night_Shift|iPhone Night Shift]]

[[Emission_Spectra_of_Different_Metals|Emission Spectra of Different Metals]]

[[Submerge_Christmas_light_in_liquid_nitrogen|Submerge Christmas light in liquid nitrogen]]

[[Laser_Cutter_Emission_Spectra|Laser cutter emission spectra]]

[[Emission_intensity_of_mercury_lamp|Emission intensity of mercury lamp]]

Laser Cutter Emission Spectra

2017-01-26T23:52:39Z

DFX: /* Intro */

==Intro==
The laser cutter emits a bright white light while cutting, this emission spectra was taken in search of a reason for the white light. The data was collected while vector cutting a circle in to a piece of wood, while the photo-diode array detector was placed in the cutting area while the laser was active to get a better angle to catch the laser in the tube as it was being sent to the cutting head.

==Graph==
[[File:LaserGraph.jpg|720px|thumb|left|Emission spectra of the laser cutter]]

Laser Cutter Emission Spectra

2017-01-26T23:47:01Z

DFX:

==Intro==
The laser cutter emits a bright white light while cutting, this emission spectra was taken in search of a reason for the white light

==Graph==
[[File:LaserGraph.jpg|720px|thumb|left|Emission spectra of the laser cutter]]

Laser Cutter Emission Spectra

2017-01-26T23:39:56Z

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==Graph==
[[File:LaserGraph.jpg|720px|thumb|left|Emission spectra of the laser cutter]]

Laser Cutter Emission Spectra

2017-01-26T23:39:37Z

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==Graph==
[[File:LaserGraph.jpg|1080px|thumb|left|Emission spectra of the laser cutter]]

Laser Cutter Emission Spectra

2017-01-26T23:39:12Z

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==Graph==
[[File:LaserGraph.jpg|400px|thumb|left|Emission spectra of the laser cutter]]

Laser Cutter Emission Spectra

2017-01-26T23:37:17Z

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==Graph==

Laser Cutter Emission Spectra

2017-01-26T23:36:29Z

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File:LaserGraph.jpg