MC Chem Wiki - User contributions [en]

CA spring 2016

2016-03-16T21:49:52Z

Celest'k: /* Update on Research work */

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''March 16th, 2016'''

Read through the Griffin system software manual for information on the column

'''March 15th, 2016'''

Updated GC-MS instrumentation page with information on the Griffin system.

'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

GC Chromatograph of Grob[http://esr.monmsci.net/wiki/index.php/File:TIC_GC_chromatograph_of_Grob.png#filelinks]

[http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf GROB info]

'''February 10th, 24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

CA spring 2016

2016-03-16T21:24:41Z

Celest'k: /* Update on Research work */

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''March 2016'''

Updated GC-MS instrumentation page.

'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

GC Chromatograph of Grob[http://esr.monmsci.net/wiki/index.php/File:TIC_GC_chromatograph_of_Grob.png#filelinks]

[http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf GROB info]

'''February 10th, 24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

Celestina Awopileda

2016-03-15T06:14:29Z

Celest'k: /* Personal Information */

==Personal Information==
Biochemistry Major '16

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Aug 2015: '''SOFIA Presentation'''

Title: Attitudes Toward STEM Introductory Courses

Nov 2015: Chem 350: '''Science Seminar'''

Title: ''Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee''

Apr 2016: Chem 350: '''Science Seminar'''

Title:

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-03-15T06:11:23Z

Celest'k: /* Presentations */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Aug 2015: '''SOFIA Presentation'''

Title: Attitudes Toward STEM Introductory Courses

Nov 2015: Chem 350: '''Science Seminar'''

Title: ''Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee''

Apr 2016: Chem 350: '''Science Seminar'''

Title:

==Interests==
Dancing

==Career Plans==

CA spring 2016

2016-03-15T06:06:09Z

Celest'k:

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''March 2016'''

'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

GC Chromatograph of Grob[http://esr.monmsci.net/wiki/index.php/File:TIC_GC_chromatograph_of_Grob.png#filelinks]

[http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf GROB info]

'''February 10th, 24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

GC-MS Electron Impact(Griffin)

2016-03-15T06:02:16Z

Celest'k:

'''Summary'''

Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical technique that combines the separation properties of gas-liquid chromatography with the detection feature of mass spectrometry to identify different substances within an unknown sample. The GC separates volatile organic compound into individual substances when heated. The heated gases are carried through a column with an inert gas such as helium.

'''Our Instrument'''

Our GC-MS system is a Griffin 450. This system includes two integrated sample introduction method: Split/splitless liquid injector for liquid sampling via direct syringe injection and Universal Sampling Port (USP) for vapor sampling by means of direct air intake through sampling line.

'''How to Use'''

GC-MS Electron Impact(Griffin)

2016-03-15T06:01:17Z

Celest'k: Created page with "Summary'''Bold text''' Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical technique that combines the separation properties of gas-liquid chromatography with the d..."

Summary'''Bold text'''

Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical technique that combines the separation properties of gas-liquid chromatography with the detection feature of mass spectrometry to identify different substances within an unknown sample. The GC separates volatile organic compound into individual substances when heated. The heated gases are carried through a column with an inert gas such as helium.

Our Instrument'''Bold text'''

Our GC-MS system is a Griffin 450. This system includes two integrated sample introduction method: Split/splitless liquid injector for liquid sampling via direct syringe injection and Universal Sampling Port (USP) for vapor sampling by means of direct air intake through sampling line.

How to Use'''Bold text'''

Instrumentation

2016-03-15T05:23:46Z

Celest'k:

The following instrumentation is available for student use in our chemistry labs.

- HPLC with UV detector (Waters Breese)

- HPLC with RI detertor (Viscotek)

- [[GC-MS Electron Impact(Griffin)]]

- [[FT-IR_(Nicolet)|FT-IR (Nicolet)]]

- [[UV-Vis_(HP_8453)|UV-Vis (HP 8453)]]

- AA (Varian)

- EPR (JOEL)

- UV-Vis Plate Reader (ThermoFisher)

- Electrochemical WaveDriver 10 (Pine Instrument)

- Fluorescence (ThermoFisher LS)

- Automated Flash (Teledyne Isco)