MC Chem Wiki - User contributions [en]

CA spring 2016

2016-02-28T22:49:18Z

Celest'K: /* Update on Research work */

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

GC Chromatograph of Grob[http://esr.monmsci.net/wiki/index.php/File:TIC_GC_chromatograph_of_Grob.png#filelinks]

[http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf GROB info]

'''February 10th, 24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

File:TIC GC chromatograph of Grob.png

2016-02-28T22:43:16Z

Celest'K:

CA spring 2016

2016-02-27T20:03:23Z

Celest'K: /* Celestina's Research Page */

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

'''February 10th, 24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

CA spring 2016

2016-02-27T20:00:58Z

Celest'K: /* Update on Research work */

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

'''February 10th, 24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

CA spring 2016

2016-02-27T20:00:23Z

Celest'K: /* Update on Research work */

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
'''February 27th, 2016'''

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

'''February 10th,24th 2016'''

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

'''January 2016'''

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

CA spring 2016

2016-02-27T19:59:41Z

Celest'K: /* Update on Research work */

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
February 27th, 2016

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols. Below is a URL for more information on Grob GC Test mixture.

http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

February 10th,24th 2016

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

January 2016

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

CA spring 2016

2016-02-27T19:51:49Z

Celest'K: /* Update on Research work */

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
February 27th, 2016

We verified the working of the chromatographic system and separation capabilities over a range of temperatures by running the Grob Mixture
containing hydrocarbons, fatty acidmethyl esters (FAMEs), acids, bases and alcohols.

http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

CA spring 2016

2016-02-27T19:47:35Z

Celest'K: /* Update on Research work */

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
February 27th, 2016

http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

CA spring 2016

2016-02-27T19:42:51Z

Celest'K:

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
February 27th, 2016
http://www.flir.com/uploadedFiles/flirGS/Threat_Detection/Chemical_Detection/Mass_Spectrometry/Products/Griffin/Griffin_824/grob%20gc%20test%20mixture_6.pdf

GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

Celestina Awopileda

2016-02-27T19:40:25Z

Celest'K: /* Presentations */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Aug 2015: '''SOFIA Presentation'''

Title: Attitudes Toward STEM Introductory Courses

Nov 2015: Chem 350: '''Science Seminar'''

Title: ''Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee''

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-02-27T19:39:51Z

Celest'K: /* Presentations */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Aug 2015: SOFIA Presentation

Title: Attitudes Toward STEM Introductory Courses

Nov 2015: Chem 350: '''Science Seminar'''

Title: ''Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee''

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-02-27T19:36:17Z

Celest'K: /* Presentations */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Nov 2015: Chem 350: '''Science Seminar'''

Title: ''Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee''

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-02-27T19:35:21Z

Celest'K: /* Presentations */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Nov 2015: Chem 350: '''Science Seminar'''
Title: Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee'''

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-02-27T19:34:40Z

Celest'K: /* Presentations */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==
Nov 2015: Chem 350: '''Science Seminar'''
Title: Gas Chromatography-Mass Spectrum Analysis of Aroma Compounds in Coffee

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-02-27T19:28:00Z

Celest'K: /* Interests */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==

==Interests==
Dancing

==Career Plans==

Celestina Awopileda

2016-02-27T19:27:18Z

Celest'K: /* Personal Information */

==Personal Information==
Senior Biochemistry Major

==Undergraduate Research Activities==

[[CA_spring_2016|Spring 2016]]

==Presentations==

==Interests==

==Career Plans==

Celestina Awopileda

2016-02-24T22:22:06Z

Celest'K:

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding and reading journal articles for writing the abstract and introduction of the paper on this project.

Celestina Awopileda

2016-02-24T22:19:50Z

Celest'K:

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==
GC-MS instrumentation experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen were made in methanol within the concentration ranges of 75-400μg/mL as suggested in the paper. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding journal articles for writing the abstract and introduction of the paper on this project.

Celestina Awopileda

2016-02-24T22:11:02Z

Celest'K:

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==

The experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen with methanol were made within the concentration ranges of 75-400μg/mL. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL) resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding journal articles for writing the abstract and introduction of the paper on this project.

Celestina Awopileda

2016-02-24T22:10:39Z

Celest'K:

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

== Update on Research work ==

The experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3μL of pyridine. 1μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen with methanol were made within the concentration ranges of 75-400μg/mL. Injections of different concentration(100μg/mL, 200μg/mL, 400μg/mL)resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding journal articles for writing the abstract and introduction of the paper on this project.

Celestina Awopileda

2016-02-24T22:04:47Z

Celest'K:

== Celestina's Research Page ==

== Proposed Project Summary ==
'''Identification of Acetaminophen Free Radical Formation Products'''

The primary goal of this project is to identify acetaminophen free radical formation products using GC-MS. The commonly used pain killer APAP is very toxic when ingested in high doses and can result in kidney and liver damage. Cytochrome P-450 is found to be one of the two enzymes that catalyze the oxidation of APAP leading to the formation of toxic metabolites and covalent modification of proteins. This project aims to analyze these metabolites. Samples are to be prepared by acetylation of APAP polymers.

Update on Research work

The experimental procedure found in a paper on determination of acetaminophen using GC-MS was followed. The GC was programmed at an initial temperature of 70°C for 1min, ramped up to 250°C at a rate of 30°C/min followed by a hold at 250°C for 20 min in splitless mode. The sample was made by acetylation of acetaminophen by adding drops of acetic anhydride and 3uL of pyridine. 1-μL of acetylated acetaminophen in methanol was injected, and there was no peak detection at all for 3 runs.

In order to get a working solution, several dilutions of stock unacetylated acetaminophen with methanol were made within the concentration ranges of 75-400ug/mL. Injections of different concentration(100ug/mL, 200ug/mL, 400ug/mL)resulted in no peak detection after 2 runs were conducted for each. Some of the data were saved even though there was no peaks observed. To further the overall research work until a working solution is developed, progress has been made in finding journal articles for writing the abstract and introduction of the paper on this project.

Celestina Awopileda

2016-02-10T03:20:24Z

Celest'K: Created page with " == Celestina's Research Page =="

== Celestina's Research Page ==