File:WATER.png

2016-04-22T15:58:48Z

Ahmadpauzi:

2016-04-07T21:00:31Z

Ahmadpauzi: /* Silicon Casting */

==Introduction to Flow Cells==
[[File:Yflow_cell.PNG|200px|thumb|right|Typical Y-Flow Cell with Laminar Flow]]

The purpose of this experiment was to see if distinguishing a flow cells mixing area would change the flow through the system. In a regular T- or Y-flow cell there is a laminar flow, a flow in which the two sides do not mix, that can be manipulated to give a non-laminar flow through the system. Using ten different manipulation of a typical flow cell, a way of having a non-laminar flow was experimented. Blue and yellow dyed RO water was used to determine the laminar/non-laminar flow of the flow cells by way of parastaltic flow pumps. These flow cells were created using Tinkercad to make a virtual object, 3D printed, and casted with silicon. To attach the parastaltic pumps to the flow cell, an acrylic plastic was drilled and tapped at the precise measurements of the flow cell created. Each flow cell was used and it was determined which resulted in laminar flow and which resulted in non-laminar flow as shown below.

==Producing The Virtual Object==
Tinkercad.com

Sujith

==Prep for 3d Printing==
[[File:Printer.PNG|200px|thumb|right|top|Select The Printer in Matter Control]]
A gcode file is required in order to print an object on the 3D printer. This file can be created by exporting one's Tinkercad object as an STL. The STL file can be imported into Matter Control.

The gcode file contains settings specific to the 3D printer being used in production of the flow cell. As a result, one must ensure that the proper printer, material, and settings are chosen before exportation of the final gcode.
{|align="center"
|[[File:Settings_Layers.PNG |200px|thumb|right|Matter Control Layer Settings]]
||[[File:Settings_Infill.PNG|200px|thumb|right|Matter Control Infill Settings]]
||[[File:Settings_Raft.PNG |200px|thumb|right|Matter Control Raft Settings]]
|-
|[[File:Settings_Support.PNG|200px|thumb|right|Matter Control Support Settings]]
||[[File:Settings_Filament_Filament.PNG|200px|thumb|right|Matter Control Filament Settings]]
||[[File:Settings_Filament_Cooling.PNG|200px|thumb|right|Matter Control Cooling Settings]]
|}

==3d Printing==

==Silicon Casting==

After the 3D- printed cast has been made, now the actual flow cell can be created. We have used a 10:1 ratio of Slygard 184 (a silicon monomer) to curating agent, or about 5 grams Slygard 184 to 0.5 grams curating agent. After this is mixed thoroughly, we pour it into the cast and place it in a desiccator and place a vacuum on it to let the air bubbles come to the surface; once the vacuum is taken off, the bubbles will pop if left long enough.

[[File:Cast in desiccator.jpg|200px|thumb|center|Matter Control Layer Settings]]

After this, we can leave the cast to curate, or let the silicon harden and shape to the cast. There were a few ways this was done. Some put their cast into the oven, but due to the low melting temperature of the acrylic plastic, they started to melt, making them unable to be reused. Others, however, left theirs out over the time span of a week to harden. A table of how they have been done is listed below.

[[File:Siliconecasting.png|600px|thumb|center|Matter Control Layer Settings]]

==Flow Cell Construction==

==Flow Cell Experimentation==
video/camera
results

{|align="center"
| Brad ||Chris||Morgan||Tyler||Ian||Kayla||Matt||Priscilla||Desyi||Sujith
|-
|1||[[File:Chris_Tinker.PNG |200px]][[File:Chris_Printed.png|200px]]||[[File:Morgan%27sVirtual.PNG|100px]][[File:MReality.PNG |100px]]||4||5||6||7||8||9||10
|-
|1||2||3||4||5||6||7||8||9||10
|}

File:Siliconecasting.png

2016-04-07T20:57:57Z

Ahmadpauzi:

File:Screen Shot 2016-04-07 at 3.51.46 PM.png

2016-04-07T20:52:40Z

Ahmadpauzi:

File:Screen Shot 2016-04-07 at 3.49.31 PM.png

2016-04-07T20:50:08Z

Ahmadpauzi:

2016-03-30T00:49:24Z

Ahmadpauzi:

Enzymatic Oxidation of Methyleugenol

Methyleugneol is a phenolic type of flavourant found in essential oils, which is primarily derived from cloves. This compound has been known to be a human carcinogen listed on the National Toxicology Program Report. Evidence suggests that Eugenol displays an extensive span of biological activities.Furthermore, studies has shown that Eugenol to be carcinogenic after long term dietary administration in animals at high doses. The mechanism of hepatic carcinogenesis resulted from the bioactivation of the Methyleugenol has also shown to occur in the liver. The mechanism behind the chemical oxidation of Eugenol is that it gives a one electron oxidation to form a Eugenol radical and the radical will recombine together in a couple of different ways to form polymers. In this study, we show that Eugenol undergoes Enzymatic oxidation with Hydrogen Peroxide and Horseradish peroxidase to form free radicals.













You have reached the personal page of Ahmad Pauzi.

==Personal Information==
Senior Chemistry Major

Hometown: Kajang, Malaysia

Contact Info: 309-371-4006

==Undergraduate Research Activities==

Summer 2015: Kieft Summer Research with Brad Sturgeon (Chemical Oxidation of Biophenols)

Spring 2016: Chem 430 with Brad Sturgeon

==Presentations==

June 2015: '''Kieft Summer Research Presentations'''

Title: Enzymatic Oxidation of Biophenols'''

Nov 2015: '''Local Section ACS Meeting''' St. Ambrose, Davenport IA.

Poster Title: ''Chemical Oxidation of Butylated Hydroxyanisole BHA''

March 2016: '''258th National ACS Meeting''' San Diego, CA

Poster Title: ''Enzymatic Oxidation of Eugenol and Methyleugenol''

April 2016: Chem 350: '''Science Seminar'''

Poster Title: ''Enzymatic Oxidation of Eugenol and Methyleugenol ''

==Interests==

Travelling

==Career Plans==

Analytical Chemist

Academician